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Modification and characterization of RGD-EV. (A) Western blot analysis of Alix, TSG101 in ReN cells and corresponding EVs; supernatant obtained from ultracentrifugation during EVs isolation was used as a negative control. (B) Schematic diagram summarizing conjugation of c(RGDyK) and Cy5.5 fluorophore to EV amine groups by a two-step reaction. (C,D) NTA (C) and TEM imaging (D) of unmodified EVs and RGD-EV; scale bar, 200 nm.
