Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 12;12:5939. doi: 10.1038/s41467-021-26189-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a Structure of l-O-phosphoserine (pSer) and its non-hydrolyzable analog l-2-Amino-4-phosphonobutyric acid (^nhpSer). b Two-dimensional heteronuclear ¹H–¹⁵N HSQC NMR spectra of pUb (colored in green) and ^nhpUb (colored in purple) are superimposed. Assigned backbone amide resonance signals from the relaxed state (m) and the retracted state (n) of ^nhpUb are labeled in the spectrum by using the one letter code for amino acids. c, d Weighted chemical shift perturbation (CSP, Δω) mappings comparing either the relaxed states (c) or the retracted states of pUb and ^nhpUb with each other (d). Δω values exceeding the horizontal straight line are larger than the mean and Δω values exceeding the horizontal dashed line are larger than the mean plus one standard deviation. Residues that could not be assigned in both Ub species are indicated by an asterisk. Secondary structure elements according to the NMR solution structure of Ub (PDB ID 1D3Z)⁷⁴ are depicted on top of the graphs.