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. 2021 Oct 12;12:5939. doi: 10.1038/s41467-021-26189-9

Fig. 6. pNEDD8 but not pUb stimulates HSP70 ATPase activity.

Fig. 6

a, b Selected proteins from the AE-MS experiments performed with HEK293T (a) or SHSY5Ycell (b) extracts with a binding preference for pUb or pNEDD8. Thresholds for the ANOVA statistics were set as follows: FDR = 0.02, S0 = 1. Enrichment is indicated in red, whereas lack of enrichment is indicated in blue. Clusters are as in Fig. 4b. c String interaction networks showing only interactions with the highest confidence (interaction score 0.9) for the proteins selected in a and b. d The relative stimulation of the ATPase activity of HSPA8, a member of the HSP70 family, was measured in presence of Ub, pUb, nhpUb, NEDD8, pNEDD8, and nhpNEDD8 and compared to HSP70 basal ATPase activity (n = 3 ± SEM independent experiments). Source data are provided as a Source Data file.