Table 1. S. pneumoniae strains used in this study.
| Strains | Relevant properties | Source or ref. |
|---|---|---|
| R6 | Wild type | [26] |
| D39 | Wild type | [34] |
| R800 | R6 rpsL1; StrR | [35] |
| ΔbceAB | R6 Δspr0812 spr0813::CatR D39 ΔSPD_0804 SPD_0805::CatR |
This work |
| Δtcs01 |
R6 Δspr1473 spr1474::KanR D39 ΔSPD_1445 SPD_1446::KanR |
This work |
| ΔbceAB/Δtcs01 | R6 Δspr0812 spr0813::CatR
/ Δspr1473 spr1474::KanR D39 ΔSPD_0804 SPD_0805::CatR / ΔSPD_1445 SPD_1446::KanR |
This work |
| ΔbceA::kan-rpsL | R800, Δspr0812::Kan-rpsL | This work |
| bceA-kan-rpsL a | R800, spr0812 Kan-rpsL | This work |
| bceA-gfp | R800, spr0812-gfp | This work |
| Δhk01::kan-rpsLa | R800, Δspr1473::Kan-rpsL | This work |
| Δhk01 | R800, Δspr1473 | This work |
| Δhk01 bceA-kan-rpsLa | R800, Δspr1473, spr0812 Kan-rpsL | This work |
| Δhk01 bceA-gfp | R800, Δspr1473, spr0812-gfp | This work |
| PcomX-kan-rpsLa | R800 rpsL::rpsL1, Δspr0565Nter::P1::PcomR-comR, cpsN-cpsO::PcomX-kan-rpsL | [36] |
| PcomX-rr01-hk01a | R800 rpsL::rpsL1, Δspr0565Nter::P1::PcomR-comR, cpsN-cpsO::PcomX-rr01-hk01 | This work |
| PcomX-rr01-hk01 Δhk01::kan-rpsLa | R800 rpsL::rpsL1, Δspr0565Nter::P1::PcomR-comR, cpsN-cpsO::PcomX-rr01-hk01, Δspr1473::kan-rpsL | This work |
| PcomX-rr01-hk01 Δhk01 | R800 rpsL::rpsL1, Δspr0565Nter::P1::PcomR-comR, cpsN-cpsO::PcomX-rr01-hk01, Δspr1473 | This work |
| PcomX-rr01-hk01 Δhk01 ΔbceAB::kan-rpsLa | R800 rpsL::rpsL1, Δspr0565Nter::P1::PcomR-comR, cpsN-cpsO::PcomX-rr01-hk01, Δspr1473, Δspr0812-spr0813::kan-rpsL | This work |
| PcomX-rr01-hk01 Δhk01 ΔbceAB | R800 rpsL::rpsL1, Δspr0565Nter::P1::PcomR-comR, cpsN-cpsO::PcomX-rr01-hk01, Δspr1473, Δspr0812-spr0813 | This work |
a These strains are intermediate constructs that were not directly used in our experiments