Table 2. Minimum Inhibitory Concentrations (μg/ml) of various antimicrobial peptides (AMPs) against the R6 and D39 strains.
R6 strain | |||||
AMPs | WT | ΔbceAB | Δtcs01 | ΔbceAB/tcs01 | Fold sensitivity displayed by the KO strains |
actagardin | 32 | 2 | 2 | 2 | 16 |
bacitracin | 8 | 1 | 1 | 1 | 8 |
nisin | 3.2 | 0.4 | 0.4 | 0.4 | 8 |
Planosporicin (NAI-97) | 16 | 2 | 2 | 2 | 8 |
NAI-802 | 16 | 2 | 2 | 2 | 8 |
Microbisporicin (NAI-107) | 0.032 | 0.008 | 0.008 | 0.008 | 4 |
NAI-857 | 4 | 2 | 2 | 2 | 2 |
gramicidin | 0.016 | 0.016 | 0.016 | 0.016 | 1 |
ramoplanin | 0.02 | 0.02 | 0.02 | 0.02 | 1 |
vancomycin | 0.5 | 0.5 | 0.5 | 0.5 | 1 |
mastoporan | 32 | 32 | 32 | 32 | 1 |
colistin | 256 | 256 | 256 | 256 | 1 |
D39 strain | |||||
AMPs | WT | ΔbceAB | Δtcs01 | ΔbceAB/tcs01 | Fold sensitivity diplayed by the KO strains |
bacitracin | 4 | 1 | 1 | 1 | 4 |
nisin | 3.2 | 0.2 | 0.2 | 0.2 | 16 |
actagardin | 32 | 2 | 2 | 2 | 16 |
vancomycin | 0.25 | 0.25 | 0.25 | 0.25 | 1 |
ramoplanin | 0.03 | 0.03 | 0.03 | 0.03 | 1 |
Experiments were performed with two to four biological replicates, each with technical duplicates.