Appendix 1—key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-nc82 (mouse monoclonal) | DSHB | Cat# nc82; RRID:AB_2314866 |
IHC (1:25) |
| Antibody | Anti-cd8 (rat monoclonal) | Thermo Fisher Scientific | Cat# 14-0081-82; RRID:AB_467087 |
IHC (1:100 or 1:200) |
| Antibody | Anti-elav (rat monoclonal) | DSHB | Cat# Rat-Elav-7E8A10; RRID:AB_528218 |
IHC (1:100) |
| Antibody | Anti-Orco (rabbit polyclonal) | Gift from Leslie Vosshall Larsson et al., 2004 | IHC (1:100) | |
| Antibody | Anti-Ir25a (rabbit polyclonal) | Gift from Benton et al., 2009 | RRID:AB_2567027 | IHC (1:100) |
| Antibody | Anti-Ir8a (guinea pig polyclonal) | Gift from Richard Benton Abuin et al., 2011 | RRID:AB_2566833 | IHC (1:100 for whole-mount, 1:1000 for cryosections) |
| Antibody | Anti-Ir64a (rabbit polyclonal) | Gift from Greg Suh Ai et al., 2010 | RRID:AB_2566854 | IHC (1:100) |
| Antibody | Anti-guinea pig Alexa 568 (goat polyclonal) | Thermo Fisher Scientific | Cat# A11075; RRID:AB_141954 |
IHC (1:500) |
| Antibody | Anti-rabbit Cy3 conjugated AffiniPure 568 (goat polyclonal) |
Jackson ImmunoResearch | Cat# 111-165-144; RRID:AB_2338006 |
IHC (1:500) |
| Antibody | Anti-mouse Cy3 (goat polyclonal) | Jackson ImmunoResearch | Cat# 115-165-166; RRID:AB_2338692 |
IHC (1:200) |
| Antibody | Anti-mouse Alexa 647 (goat polyclonal) | Jackson ImmunoResearch | Cat# 115-605-166; RRID:AB_2338914 |
IHC (1:200) |
| Antibody | Anti-guinea pig Cy3 (donkey polyclonal) | Jackson ImmunoResearch | Cat# 706-165-148; RRID:AB_2340460 |
IHC (1:200) |
| Antibody | Anti-rat Cy3 (goat polyclonal) | Jackson ImmunoResearch | Cat# 112-165-167; RRID:AB_2338251 |
IHC (1:200) |
| Antibody | Anti-rat Alexa 647 (goat polyclonal) | Jackson ImmunoResearch | Cat# 112-605-167; RRID:AB_2338404 |
IHC (1:200) |
| Antibody | Anti-rabbit Cy3 (goat polyclonal) | Jackson ImmunoResearch | Cat# 111-165-144; RRID:AB_2338006 |
IHC (1:200) |
| Antibody | Anti-rabbit Alexa 647 (goat polyclonal) | Jackson ImmunoResearch | Cat# 111-605-144; RRID:AB_2338078 |
IHC (1:200) |
| Recombinant DNA reagent | pHACK-QF2 (plasmid) | Addgene Lin and Potter, 2016a | Plasmid# 80274; RRID:Addgene_80274 |
QF2 HACK backbone Contains QF2-hsp70, but no gRNAs |
| Recombinant DNA reagent | p10XQUAS-CsChrimson-SV40 (plasmid) | This paper | Plasmid# 163629; RRID:Addgene_163629 |
For red-shifted optogenetic activation of neurons under control of the Q-system; see ’Fly stocks’ |
| Recombinant DNA reagent | pHACK-QF2Orco (plasmid) | This paper | HACK construct targeting the Orco gene; see ‘Plasmid construction’ | |
| Recombinant DNA reagent | pHACK-QF2Ir8a (plasmid) | This paper | HACK construct targeting the Ir8a gene; see ‘Plasmid construction’ | |
| Recombinant DNA reagent | pHACK-QF2Ir76b (plasmid) | This paper | HACK construct targeting the Ir76b gene; see ‘Plasmid construction’ | |
| Recombinant DNA reagent | pHACK-QF2Ir25a (plasmid) | This paper | HACK construct targeting the Ir25a gene; see ‘Plasmid construction’ | |
| Genetic reagent (Drosophila melanogaster) | Orco-T2A-QF2 knock-in | This paper | BDSC 92400, 92401, 92402 | See ‘Generation of HACK knock-in lines’ |
| Genetic reagent (D. melanogaster) | Ir8a-T2A-QF2 knock-in | This paper | BDSC 92398, 92399 | See ‘Generation of HACK knock-in lines’ |
| Genetic reagent (D. melanogaster) | Ir76b-T2A-QF2 knock-in | This paper | BDSC 92396, 92397 | See ‘Generation of HACK knock-in lines’ |
| Genetic reagent (D. melanogaster) | Ir25a-T2A-QF2 knock-in | This paper | BDSC 92392, 92393, 92394, 92395 |
See ‘Generation of HACK knock-in lines’ |
| Genetic reagent (D. melanogaster) | 10XQUAS-CsChrimson (y[1] w[*]; Pin[1]/CyO; P(w[+mC] = 10XQUAS-CsChrimson.mVenus)11c) | This paper | BDSC 91996, FlyBase FBst0091996 |
See ‘Fly stocks’ |
| Genetic reagent (D. melanogaster) | Ir21a-T2A-Gal4 knock-in | Gift from Paul Garrity Marin et al., 2020 | ||
| Genetic reagent (D. melanogaster) | Ir68a-T2A-Gal4 knock-in | Gift from Paul Garrity Marin et al., 2020 | ||
| Genetic reagent (D. melanogaster) | Or7a-Gal4 knock-in (y[1] w[*] TI(GAL4)Or7a[KI-GAL4.w-]) | Potter lab | BDSC 91991, FlyBase FBti0214362 |
|
| Genetic reagent (D. melanogaster) | Ir64a-Gal4 | Gift from Greg Suh lab Ai et al., 2010 | ||
| Genetic reagent (D. melanogaster) | Rh50-Gal4 | Menuz lab Vulpe et al., 2021 | ||
| Genetic reagent (D. melanogaster) | Amt-Gal4 | Menuz lab Menuz et al., 2014 | ||
| Genetic reagent (D. melanogaster) | Repo-Gal80 | Awasaki et al., 2011 | FlyBase FBtp0067904 | |
| Genetic reagent (D. melanogaster) | wCS (Cantonized w1118) | Koh et al., 2014 | FlyBase FBrf0226011 | |
| Genetic reagent (D. melanogaster) | Or35a-Gal4 | Yao et al., 2005 | ||
| Genetic reagent (D. melanogaster) | Crey +1B (y[1] w[67c23] P(y[+mDint2] = Crey)1b; sna[Sco]/CyO) | Bloomington Drosophila Stock Center | BDSC 766, FlyBase FBti0012692 |
|
| Genetic reagent (D. melanogaster) | Gr21a-GAL4 (w[*]; P(w[ + mC] = Gr21a-GAL4.9.323)2/CyO; Dr[1]/TM3, Sb[+]) | Bloomington Drosophila Stock Center | BDSC 57600, FlyBase FBti0162643 |
|
| Genetic reagent (D. melanogaster) | Gr28b.d-GAL4 (w[*]; P(w[+mC] = Gr28b.d-GAL4)B27; Dr[1]/TM3, Sb[1]) | Bloomington Drosophila Stock Center | BDSC 57620, FlyBase FBst0057620 |
|
| Genetic reagent (D. melanogaster) | Ir25a-GAL4 (w[*]; P(w[ + mC] = Ir25a-GAL4.A)236.1; TM2/TM6B, Tb[1]) | Bloomington Drosophila Stock Center | BDSC 41728, FlyBase FBti0148895 |
|
| Genetic reagent (D. melanogaster) | Ir40a-GAL4 (w[*]; P(w[ + mC] = Ir40a-GAL4.3011)214.1; TM2/TM6B, Tb[1]) | Bloomington Drosophila Stock Center | BDSC 41727, FlyBase FBst0041727 |
|
| Genetic reagent (D. melanogaster) | Ir41a-GAL4 (w[*]; P(y[+ t7.7] w[+mC] = Ir41a-GAL4.2474)attP40; TM2/TM6B, Tb[1]) | Bloomington Drosophila Stock Center | BDSC 41749, FlyBase FBst0041749 | |
| Genetic reagent (D. melanogaster) | Ir64a-GAL4 (w[*]; P(w[+mC] = Ir64a-GAL4.A)183.8; TM2/TM6B, Tb[1]) | Bloomington Drosophila Stock Center | BDSC 41732, FlyBase FBti0148898 | |
| Genetic reagent (D. melanogaster) | Ir76a-GAL4 (w[*]; P(w[+ mC] = Ir76a-GAL4.PB)292.3B; TM2/TM6B, Tb[1]) | Bloomington Drosophila Stock Center | BDSC 41735, FlyBase FBst0041735 | |
| Genetic reagent (D. melanogaster) | Ir76b-GAL4 (w[*]; P(w[ + mC] = Ir76b-GAL4.916)226.8; TM2/TM6B, Tb[+]) | Bloomington Drosophila Stock Center | BDSC 41730, FlyBase FBti0153291 | |
| Genetic reagent (D. melanogaster) | Ir8a-GAL4 (w[*]; P(w[ + mC] = Ir8a-GAL4.A)204.8; TM2/TM6B, Tb[1]) | Bloomington Drosophila Stock Center | BDSC 41731, FlyBase FBti0148897 | |
| Genetic reagent (D. melanogaster) | Or10a-Gal4 (w[*]; l(2)*[*]/CyO; P(w[ + mC] = Or10a-GAL4.C)134t1.3) | Bloomington Drosophila Stock Center | BDSC 23885, FlyBase FBti0102042 | |
| Genetic reagent (D. melanogaster) | Or13a-Gal4 (w[*]; P(w[ + mC] = Or13a-GAL4.C)229t56.2/TM3, Sb[1]) | Bloomington Drosophila Stock Center | BDSC 23886, FlyBase FBti0102056 | |
| Genetic reagent (D. melanogaster) | Or22a-Gal4 (w[*]; P(w[ + mC] = Or22a-GAL4.7.717)14.2) | Bloomington Drosophila Stock Center | BDSC 9951, FlyBase FBti0101805 | |
| Genetic reagent (D. melanogaster) | Or22a-Gal4 (w[*]; P(w[ + mC] = Or22a-GAL4.7.717)14.21) | Bloomington Drosophila Stock Center | BDSC 9952, FlyBase FBti0101805 | |
| Genetic reagent (D. melanogaster) | Or33c-Gal4 (w[*]; P(w[ + mC] = Or33c-GAL4.F)78.3) | Bloomington Drosophila Stock Center | BDSC 9966, FlyBase FBti0101843 | |
| Genetic reagent (D. melanogaster) | Or35a-cd8GFP (w[*]; P(w[ + mC] = Or35a-Mmus\Cd8a.GFP)3/TM3, Sb[1]) | Bloomington Drosophila Stock Center | BDSC 52624, FlyBase FBti0156834 | |
| Genetic reagent (D. melanogaster) | Or35a-Gal4 (w[*]; P(w[ + mC] = Or35a-GAL4.F)109.2A) | Bloomington Drosophila Stock Center | BDSC 9967, FlyBase FBti0101810 | |
| Genetic reagent (D. melanogaster) | Or35a-Gal4 (w[*]; P(w[ + mC] = Or35a-GAL4.F)109.3) | Bloomington Drosophila Stock Center | BDSC 9968, FlyBase FBti0101844 | |
| Genetic reagent (D. melanogaster) | Or42a-Gal4 (w[*]; P(w[ + mC] = Or42a-GAL4.F)48.3B) | Bloomington Drosophila Stock Center | BDSC 9970, FlyBase FBti0101811 | |
| Genetic reagent (D. melanogaster) | Or42b-Gal4 (w[*]; P(w[ + mC] = Or42b-GAL4.F)64.3) | Bloomington Drosophila Stock Center | BDSC 9971, FlyBase FBti0101812 | |
| Genetic reagent (D. melanogaster) | Or43b-Gal4 (w[*]; P(w[ + mC] = Or43b-GAL4.C)110t8.1) | Bloomington Drosophila Stock Center | BDSC 23894, FlyBase FBti0102047 | |
| Genetic reagent (D. melanogaster) | Or46a-Gal4 (w[1,118]; P(w[ + mC] = Or46a-GAL4.G)32.1.y) | Bloomington Drosophila Stock Center | BDSC 23291, FlyBase FBti0076800 | |
| Genetic reagent (D. melanogaster) | Or47a-Gal4 (w[*]; P(w[ + mC] = Or47a-GAL4.8.239)15.4A) | Bloomington Drosophila Stock Center | BDSC 9982, FlyBase FBti0101851 | |
| Genetic reagent (D. melanogaster) | Or49b-Gal4 (w[*]; P(w[ + mC] = Or49b-GAL4.F)80.1) | Bloomington Drosophila Stock Center | BDSC 9986, FlyBase FBti0101853 | |
| Genetic reagent (D. melanogaster) | Or59b-Gal4 (w[*]; P(w[ + mC] = Or59b-GAL4.C)114t2.2) | Bloomington Drosophila Stock Center | BDSC 23897, FlyBase FBti0102060 | |
| Genetic reagent (D. melanogaster) | Or59c-Gal4 (w[*]; P(w[ + mC] = Or59c-GAL4.C)129t1.1) | Bloomington Drosophila Stock Center | BDSC 23899, FlyBase FBti0102061 | |
| Genetic reagent (D. melanogaster) | Or65a-Gal4 (w[*]; Bl[1]/SM1; P(w[ + mC] = Or65a-GAL4.F)72.1) | Bloomington Drosophila Stock Center | BDSC 9994, FlyBase FBti0101857 | |
| Genetic reagent (D. melanogaster) | Or67a-Gal4 (w[*]; P(w[ + mC] = Or67a-GAL4.C)137t3.3) | Bloomington Drosophila Stock Center | BDSC 23904, FlyBase FBti0102049 | |
| Genetic reagent (D. melanogaster) | Or67b-Gal4 (w[*]; P(w[ + mC] = Or67b-GAL4.F)68.3/TM6B, Tb[1]) | Bloomington Drosophila Stock Center | BDSC 9995, FlyBase FBti0101858 | |
| Genetic reagent (D. melanogaster) | Or67c-Gal4 (w[*]; P(w[ + mC] = Or67c-GAL4.C)116t3.2/CyO) | Bloomington Drosophila Stock Center | BDSC 23905, FlyBase FBti0102050 | |
| Genetic reagent (D. melanogaster) | Or71a-Gal4 (w[*]; P(w[ + mC] = Or71a-GAL4.F)30.4) | Bloomington Drosophila Stock Center | BDSC 23122, FlyBase FBti0101860 | |
| Genetic reagent (D. melanogaster) | Or83c-Gal4 (w[*]; P(w[ + mC] = Or83c-GAL4.F)73.3B) | Bloomington Drosophila Stock Center | BDSC 23131, FlyBase FBti0101829 | |
| Genetic reagent (D. melanogaster) | Or85a-Gal4 (w[*]; P(w[ + mC] = Or85a-GAL4.F)67.2) | Bloomington Drosophila Stock Center | BDSC 23133, FlyBase FBti0101830 | |
| Genetic reagent (D. melanogaster) | Or85b-Gal4 (w[*]; P(w[ + mC] = Or85b-GAL4.C)179t5.1) | Bloomington Drosophila Stock Center | BDSC 23911, FlyBase FBti0102053 | |
| Genetic reagent (D. melanogaster) | Or85d-Gal4 (w[*]; P(w[ + mC] = Or85d-GAL4.C)143t2.1) | Bloomington Drosophila Stock Center | BDSC 24148, FlyBase FBti0102066 | |
| Genetic reagent (D. melanogaster) | Or92a-Gal4 (w[*]; P(w[ + mC] = Or92a-GAL4.F)62.1) | Bloomington Drosophila Stock Center | BDSC 23139, FlyBase FBti0101867 | |
| Genetic reagent (D. melanogaster) | Or98a-Gal4 (w[*]; P(w[ + mC] = Or98a-GAL4.F)115.1) | Bloomington Drosophila Stock Center | BDSC 23141, FlyBase FBti0101868 | |
| Genetic reagent (D. melanogaster) | Orco-Gal4 (w[*]; P(w[ + mC] = Orco-GAL4.W)11.17; TM2/TM6B, Tb[1]) | Bloomington Drosophila Stock Center | BDSC 26818, FlyBase FBti0101150 | |
| Genetic reagent (D. melanogaster) | QUAS reporter (y[1] w[1,118]; P(w[ + mC] = QUAS-mCD8::GFP.P)5B/TM6B, Tb[1]) | Bloomington Drosophila Stock Center | BDSC 30003, FlyBase FBti0129937 | |
| Genetic reagent (D. melanogaster) | QUAS reporter (y[1] w[*]; PBac(y[ + mDint2] w[ + mC] = 10XQUAS-6XGFP)VK00018/CyO, P(Wee-P.ph0)Bacc[Wee-P20]) | Bloomington Drosophila Stock Center | BDSC 52264, FlyBase FBti0162759 | |
| Genetic reagent (D. melanogaster) | UAS reporter (w[*]; P(y[ + t7.7] w[ + mC] = 10XUAS-IVS-mCD8::GFP)attP40) | Bloomington Drosophila Stock Center | BDSC 32186, FlyBase FBti0131963 | |
| Genetic reagent (D. melanogaster) | UAS reporter (w[*]; P(y[ + t7.7] w[ + mC] = 15XUAS-IVS-mCD8::GFP)attP2) | Bloomington Drosophila Stock Center | BDSC 32193, FlyBase FBti0131935 | |
| Genetic reagent (D. melanogaster) | UAS reporter (w[*]; P(y[ + t7.7] w[ + mC] = 10XUAS-IVS-mCD8::RFP)attP2) | Bloomington Drosophila Stock Center | BDSC 32218, FlyBase FBti0131950 | |
| Genetic reagent (D. melanogaster) | UAS reporter (w[*]; P(y[ + t7.7] w[ + mC] = 10XUAS-IVS-mCD8::RFP)attP40) | Bloomington Drosophila Stock Center | BDSC 32219, FlyBase FBti0131967 | |
| Genetic reagent (D. melanogaster) | UAS reporter (y[1] w[*]; PBac(y[ + mDint2] w[ + mC] = 20XUAS-6XGFP)VK00018/CyO, P(Wee-P.ph0)Bacc[Wee-P20]) | Bloomington Drosophila Stock Center | BDSC 52261, FlyBase FBti0162758 | |
| Genetic reagent (D. melanogaster) | UAS reporter, sacculus experiments (UAS-mCD8::GFP (2nd)) | Lee and Luo, 1999 | FlyBase FBti0012685 | |
| Genetic reagent (D. melanogaster) | UAS reporter, sacculus experiments (UAS-mCD8::GFP (3rd)) | Lee and Luo, 1999 | FlyBase FBti0012686 | |
| Genetic reagent (D. melanogaster) | Orco2 mutant (w[*]; TI(w[ + m*] = TI)Orco[2]) | Bloomington Drosophila Stock Center | BDSC 23130, FlyBase FBti0168777 | |
| Genetic reagent (D. melanogaster) | Ir25a2 mutant (w[*]; TI(w[ + m*] = TI)Ir25a[2]/CyO) | Bloomington Drosophila Stock Center | BDSC 41737, FlyBase FBti0168524 | |
| Genetic reagent (D. melanogaster) | Ir8a1 mutant (w[*] TI(w[ + mW.hs] = TI)Ir8a[1]; Bl[1] L[2]/CyO) | Bloomington Drosophila Stock Center | BDSC 41744, FlyBase FBst0041744 | |
| Genetic reagent (D. melanogaster) | Ir76b1 mutant (w[*]; Ir76b[1]) | Bloomington Drosophila Stock Center | BDSC 51309, FlyBase FBst0051309 | |
| Genetic reagent (D. melanogaster) | Germline Cas9 (y[1] M(RFP[3xP3.PB] GFP[E.3xP3] = vas-Cas9)ZH-2A w[1,118]/FM7c) | Bloomington Drosophila Stock Center | BDSC 51323, FlyBase FBti0154823 | |
| Genetic reagent (D. melanogaster) | Germline Cas9 (w[1,118]; PBac(y[ + mDint2] = vas-Cas9)VK00027) | Bloomington Drosophila Stock Center | BDSC 51324, FlyBase FBti0154822 | |
| Genetic reagent (D. melanogaster) | Germline Cas9 (y[1] M(w[ + mC] = Act5C-Cas9.P)ZH-2A w[*]) | Bloomington Drosophila Stock Center | BDSC 54590, FlyBase FBti0159182 | |
| Genetic reagent (D. melanogaster) | Double balancer (19ADrok/FM7c; Pin/CyO) | Potter lab stock | Derived from BDSC 6666, FBba0000009, FBal0013831, FBba0000025 |
|
| Genetic reagent (D. melanogaster) | Double balancer (y,w; Pin/CyO; Dh/TM6B) | Potter lab stock | Derived from FBal0013831, FBba0000025, FBti0004009, FBba0000057, FBal0016730 |
|
| Genetic reagent (D. melanogaster) | Double balancer (y,w; S/CyO; Pr/TM6B) | Potter lab stock | Derived from FBal0015108, FBba0000025, FBal0013944, FBba0000057, FBal0016730 |
|
| Genetic reagent (D. melanogaster) | Single balancer (y,w; +/+; Pr/TM6B) | Potter lab stock | Derived from FBal0013944, FBba0000057, FBal0016730 |
|
| Genetic reagent (D. melanogaster) | Single balancer (y,w; S/CyO; +/+) | Potter lab stock | Derived from FBal0015108, FBba0000025 |
|
| Genetic reagent (D. melanogaster) | Wildtype (w1118 IsoD1) | Gift from Thomas R. Clandinin | Derived from FBal0018186 | |
| Genetic reagent (Drosophila sechellia) | Wildtype, genome Cousin Island, Seychelles | National Drosophila Species Stock Center | SKU:14021-0248.25 | |
| Genetic reagent (Anopheles coluzzii) | Wildtype, N’Gousso strain | Gift from Insect Transformation Facility (Rockville, MD) Riabinina et al., 2016 | ||
| Chemical compound, drug | Mineral oil | Sigma-Aldrich | CAS# 8042-47-5, Cat# 330779-1L | |
| Chemical compound, drug | Ethyl acetate | Sigma-Aldrich | CAS# 141-78-6, Cat# 650528-1L, Cat# 270989-100ML |
|
| Chemical compound, drug | Pentyl acetate | Sigma-Aldrich | CAS# 628-63-7, Cat# 109584-250ML |
|
| Chemical compound, drug | Benzaldehyde | Sigma-Aldrich | CAS# 100-52-7, Cat# 418099-100ML, Cat# B1334-100G |
|
| Chemical compound, drug | Ethyl butyrate | Sigma-Aldrich | CAS# 105-54-4, Cat# E15701-500M, Cat# E15701-25ML |
|
| Chemical compound, drug | Hexanol | Sigma-Aldrich | CAS# 111-27-3, Cat# H13303-100ML |
|
| Chemical compound, drug | E2-hexenal | Sigma-Aldrich | CAS# 6728-26-3, Cat# W256005-1KG-K |
|
| Chemical compound, drug | Geranyl acetate | Sigma-Aldrich | CAS# 105-87-3, Cat# 173495-25G, Cat# 45896-1ML-F |
|
| Chemical compound, drug | 2-Heptanone | Sigma-Aldrich | CAS# 110-43-0, Cat# 537683-100ML |
|
| Chemical compound, drug | 1-Octen-3-ol | Sigma-Aldrich | CAS# 3391-86-4, Cat# O5284-25G, Cat# W280518-SAMPLE-K |
|
| Chemical compound, drug | 2,3-Butanedione | Sigma-Aldrich | CAS# 431-03-8, Cat# 11038-1ML-F |
|
| Chemical compound, drug | Phenylacetaldehyde | Sigma-Aldrich | CAS# 122-78-1, Cat# 107395-100ML |
|
| Chemical compound, drug | Phenethylamine | Sigma-Aldrich | CAS# 64-04-0, Cat# 241008-50ML |
|
| Chemical compound, drug | Propionic acid | Sigma-Aldrich | CAS# 79-09-4, Cat# 402907-100ML |
|
| Chemical compound, drug | 1,4-Diaminobutane | Sigma-Aldrich | CAS# 110-60-1, Cat# D13208-100G |
|
| Chemical compound, drug | Pyrrolidine | Sigma-Aldrich | CAS# 123-75-1, Cat# P73803-100ML, Cat# P73803-5ML |
|
| Chemical compound, drug | P-cresol | Sigma-Aldrich | CAS# 106-44-5, Cat# 42429-5G-F, Cat# C85751-5G |
|
| Chemical compound, drug | Methyl salicylate | Sigma-Aldrich | CAS# 119-36-8, Cat# M6752-250ML |
|
| Chemical compound, drug | Paraffin Oil | ACROS Organics | CAS# 8012-95-1, Cat# 171400010 | |
| Chemical compound, drug | Water | SIGMA Life Science | CAS# 7732-18-5, Cat# W3500 |
|
| Chemical compound, drug | Acetic acid | Fisher Scientific | CAS# 64-19-7, Cat# A38S | |
| Chemical compound, drug | Ammonium hydroxide | Fisher Scientific | CAS# 7664-41-7, Cat# A669S | |
| Chemical compound, drug | Cadaverine | Sigma-Aldrich | CAS# 462-94-2, Cat# 33211-10ML-F | |
| Chemical compound, drug | Hexanol | ACROS Organics | CAS# 111-27-3, Cat# AC43386 | |
| Chemical compound, drug | 2,3-Butanedione | ACROS Organics | CAS# 431-03-8, Cat# AC10765 | |
| Chemical compound, drug | Phenethylamine | ACROS Organics | CAS# 64-04-0, Cat# AC156491000 | |
| Chemical compound, drug | Propanal | ACROS Organics | CAS# 123-38-6, Cat# AC220511000 | |
| Chemical compound, drug | 1,4-Diaminobutane | ACROS Organics | CAS# 110-60-1, Cat# AC11212-250 | |
| Chemical compound, drug | All trans-retinal | Sigma-Aldrich | CAS# 116-31-4, Cat# R2500 | |
| Commercial assay or kit | HCR v3.0 | Molecular Instruments | ||
| Commercial assay or kit | In-Fusion Cloning | Clontech Labs | Cat# 639645 | |
| Commercial assay or kit | DNeasy Blood and Tissue Kit | QIAGEN | Cat# 69506 | |
| Software, algorithm | Fiji (ImageJ) | Schindelin et al., 2012 | https://imagej.net/Fiji | |
| Software, algorithm | GraphPad Prism 8.0 | GraphPad Software | http://www.graphpad.com/ | |
| Software, algorithm | AutoSpike | Syntech | http://www.ockenfels-syntech.com/products/signal-acquisition-systems-2/ | |
| Software, algorithm | LabChart Pro v8 | ADInstruments | https://www.adinstruments.com/support/downloads/windows/labchart | |
| Software, algorithm | Adobe Illustrator CS6 | Adobe, Inc | https://www.adobe.com/products/illustrator.html | |
| Software, algorithm | Adobe Photoshop CS6 | Adobe, Inc | https://www.adobe.com/products/photoshop.html | |
| Software, algorithm | MacVector 16.0 | MacVector, Inc | https://macvector.com/ | |
| Software, algorithm | MEGA11 | Tamura et al., 2021 | https://www.megasoftware.net/ | |
| Software, algorithm | Zen Black | Carl Zeiss Microscopy | https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html | |
| Software, algorithm | Venn Diagram web tool | VIB/UGent Bioinformatics & Evolutionary Genomics | http://bioinformatics.psb.ugent.be/webtools/Venn/ | |
| Software, algorithm | Amira | Thermo Fisher Scientific | https://www.thermofisher.com/us/en/home/industrial/electron-microscopy/electron-microscopy-instruments-workflow-solutions/3d-visualization-analysis-software/amira-life-sciences-biomedical.html | |
| Software, algorithm | Python packages | Schlegel et al., 2021 | https://github.com/schlegelp/navis, https://github.com/flyconnectome/fafbseg-py | |
| Software, algorithm | Q-Capture Pro 7 | Teledyne QImaging | https://www.qimaging.com/home | |
| Other | DAPI stain | Invitrogen | D1306 | IHC (1:10,000) |
| Sequence-based reagent | Orco_gRNA_FOR | This paper, Integrated DNA Technologies | PCR primers | TCCGGGTGAACT TCGCACAGTGCG GAGGGGGCAAG TTTTAGAGCT AGAAATAGCAAGTTA |
| Sequence-based reagent | Orco_gRNA_REV | This paper, Integrated DNA Technologies | PCR primers | TTCTAGCTCTAAAAC ACTTTATGGTGCT GGTGCAGCGACGTTA AATTGAAAATAGGTC |
| Sequence-based reagent | Orco_5HA_FOR | This paper, Integrated DNA Technologies | PCR primers | CCCTTACGTAACGCGTCAGCTT GTTTGACTTACTTGATTAC |
| Sequence-based reagent | Orco_5HA_REV | This paper, Integrated DNA Technologies | PCR primers | CGCGGCCCTCACGCGTCTTGA GCTGTACAAGTACCATAAAGT |
| Sequence-based reagent | Orco_3HA_FOR | This paper, Integrated DNA Technologies | PCR primers | GTTATAGATCACTAGTCTCAG TACTATGCAACCAGCAATA |
| Sequence-based reagent | Orco_3HA_REV | This paper, Integrated DNA Technologies | PCR primers | AATTCAGATCACTAGTGTTTT ATGAAAGCTGCAAGAAATAA |
| Sequence-based reagent | Ir8a_gRNA_FOR | This paper, Integrated DNA Technologies | PCR primers | TCCGGGTGAACTTCGTTTGT TTGTTCGGCCATGTGTTTTAG AGCTAGAAATAGCAAGTTA |
| Sequence-based reagent | Ir8a_gRNA_REV | This paper, Integrated DNA Technologies | PCR primers | TTCTAGCTCTAAAACCTGTGG GAGTCAGAGGCACCGACGT TAAATTGAAAATAGGTC |
| Sequence-based reagent | Ir8a_5HA_FOR | This paper, Integrated DNA Technologies | PCR primers | CCCTTACGTAACGCGtCTATT GGCTATTCGTCGTACTCATGC |
| Sequence-based reagent | Ir8a_5HA_REV | This paper, Integrated DNA Technologies | PCR primers | CGCGGCCCTCACGCGtCTCCA TGTAGCCACTATGTGAGTCAGAT |
| Sequence-based reagent | Ir8a_3HA_FOR | This paper, Integrated DNA Technologies | PCR primers | GTTATAGATCACTAGtGTTTCTT GTCGCACCTAATTAACAAGTG |
| Sequence-based reagent | Ir8a_3HA_REV | This paper, Integrated DNA Technologies | PCR primers | AATTCAGATCACTAGtCATAC TTAAGCTCCTTGAGGTCCAGC |
| Sequence-based reagent | Ir76b_gRNA_FOR | This paper, Integrated DNA Technologies | PCR primers | TCCGGGTGAACTTCGCAGT GATGCGAACTTCATAGTTTT AGAGCTAGAAATAGCAAGTTA |
| Sequence-based reagent | Ir76b_gRNA_REV | This paper, Integrated DNA Technologies | PCR primers | TTCTAGCTCTAAAACCGGCG GCCCTCTTTCAATACGACG TTAAATTGAAAATAGGTC |
| Sequence-based reagent | Ir76b_5HA_FOR | This paper, Integrated DNA Technologies | PCR primers | CCCTTACGTAACGCGtACCAATG AATCCTTGTCCATGCTAAA |
| Sequence-based reagent | Ir76b_5HA_REV | This paper, Integrated DNA Technologies | PCR primers | CGCGGCCCTCACGCGtCTC GGTGTAGCTGTCTTGAAGGAA |
| Sequence-based reagent | Ir76b_3HA_FOR | This paper, Integrated DNA Technologies | PCR primers | GTTATAGATC ACTAGtGCCTA ATTGGAATACC TTCTACATAATGGA |
| Sequence-based reagent | Ir76b_3HA_REV | This paper, Integrated DNA Technologies | PCR primers | AATTCAGATCACTAGtGGCAA GGCACAAAATAAAACGAAG |
| Sequence-based reagent | Ir25a_gRNA_FOR | This paper, Integrated DNA Technologies | PCR primers | TCCGGGTGAACTTCGCCGGA TACTGATTAAAGCGGTTTTA GAGCTAGAAATAGCAAGTTA |
| Sequence-based reagent | Ir25a_gRNA_REV | This paper, Integrated DNA Technologies | PCR primers | TTCTAGCTCTAAAACAGTG CTCATTTTACCATAATCGA CGTTAAATTGAAAATAGGTC |
| Sequence-based reagent | Ir25a_5HA_FOR | This paper, Integrated DNA Technologies | PCR primers | CCCTTACGTAACGCGtTGC ATGACTTCATTGACACCTCAAG |
| Sequence-based reagent | Ir25a_5HA_REV | This paper, Integrated DNA Technologies | PCR primers | CGCGGCCCTCACGCGtGAA ACGAGGCTTAAACGTAGCTGGATATT |
| Sequence-based reagent | Ir25a_3HA_FOR | This paper, Integrated DNA Technologies | PCR primers | GTTATAGATCACTAGtAATAT TATGGTTAAGTGAGCTCTCGG |
| Sequence-based reagent | Ir25a_3HA_REV | This paper, Integrated DNA Technologies | PCR primers | AATTCAGATCACTAGtCAAA GCTAAGTTCATCGTCATAGAGAC |
| Sequence-based reagent | Orco_Seq_FOR | This paper, Integrated DNA Technologies | PCR primers | GATGTTCTGCTCTTGGCTGATATTC |
| Sequence-based reagent | Ir8a_Seq_FOR | This paper, Integrated DNA Technologies | PCR primers | CATCGACTTCATCATCAGGCTTTCG |
| Sequence-based reagent | Ir76b_Seq_FOR | This paper, Integrated DNA Technologies | PCR primers | CAACGATATCCTCACGAAGAACAAGC |
| Sequence-based reagent | Ir25a_Seq_FOR | This paper, Integrated DNA Technologies | PCR primers | CGAAAGGATACAAAGGATACTGCAT |
| Sequence-based reagent | HACK_Seq_REV | This paper, Integrated DNA Technologies | PCR primers | TGTATTCCGTCGCATTTCTCTC |
| Sequence-based reagent | IVS-FOR | This paper, Integrated DNA Technologies | PCR primers | TGGGTTGGACTCAGGGAATA GATCTAAAAGGTAGGTTCAACCACT |
| Sequence-based reagent | EcoRI-SV40-REV | This paper, Integrated DNA Technologies | PCR primers | GCTTACGTCAGAATTCAGA TCGATCCAGACATGATAAGA |
| Sequence-based reagent | Tsp42Ej_FOR | This paper, Integrated DNA Technologies | PCR primers | GAGAAGTCGTTTCCCATAACACCCT |
| Sequence-based reagent | Tsp42Ej_REV | This paper, Integrated DNA Technologies | PCR primers | GAGGAGCAGTTTTCGGAGTCGCCTTC |
| Sequence-based reagent | D. melanogaster Orco | This paper, Molecular Instruments | RNA in situ HCR probe set | See Appendix 2 ‘In situ probe sequences’ |
| Sequence-based reagent | D. melanogaster Ir25a | This paper, Molecular Instruments | RNA in situ HCR probe set | See Appendix 2 ‘In situ probe sequences’ |
| Sequence-based reagent | D. melanogaster Ir40a | This paper, Molecular Instruments | RNA in situ HCR probe set | See Appendix 2 ‘In situ probe sequences’ |
| Sequence-based reagent | D. melanogaster Ir51a | This paper, Molecular Instruments | RNA in situ HCR probe set | See Appendix 2 ‘In situ probe sequences’ |
| Sequence-based reagent | D. melanogaster Ir60a | This paper, Molecular Instruments | RNA in situ HCR probe set | See Appendix 2 ‘In situ probe sequences’ |
| Sequence-based reagent | D. melanogaster Ir62a | This paper, Molecular Instruments | RNA in situ HCR probe set | See Appendix 2 ‘In situ probe sequences’ |
| Sequence-based reagent | D. melanogaster Ir76a | This paper, Molecular Instruments | RNA in situ HCR probe set | See Appendix 2 ‘In situ probe sequences’ |
| Sequence-based reagent | D. melanogaster Ir76b | This paper, Molecular Instruments | RNA in situ HCR probe set | See Appendix 2 ‘In situ probe sequences’ |
| Sequence-based reagent | D. melanogaster Ir93a | This paper, Molecular Instruments | RNA in situ HCR probe set | See Appendix 2 ‘In situ probe sequences’ |
| Sequence-based reagent | Anopheles coluzzii Orco | This paper, Molecular Instruments | RNA in situ HCR probe set | See Appendix 2 ‘In situ probe sequences’ |
| Sequence-based reagent | A. coluzzii Ir25a | This paper, Molecular Instruments | RNA in situ HCR probe set | See Appendix 2 ‘In situ probe sequences’ |