Table 1. Summary of HACK knock-in efficiency (related to Figure 2).
Gene | Approach | mCherry+ | mCherry- | Total | Efficiency (%) | Founders producing knock-in (#/total) | Knock-ins sampled | False positives | Confirmed | Correct (%) |
---|---|---|---|---|---|---|---|---|---|---|
Orco | Direct injection | 180 | 365 | 545 | 33 | 43% (3/7) | 30 | 0 | 30 | 100 |
Ir8a | Direct injection | 53 | 609 | 662 | 8 | 20% (4/20) | 5 | 0 | 5 | 100 |
Ir76b | Direct injection | 79 | 184 | 263 | 30 | 100% (2/2) | 10 | 0 | 10 | 100 |
Ir25a | Direct injection | 82 | 268 | 350 | 23 | 40% (2/5) | 6 | 0 | 6 | 100 |
Orco | Cross | 37 | 96 | 133 | 28 | 100% (3/3) | 2 | 1 | 1 | 50 |
Ir25a | Cross | 30 | 95 | 125 | 24 | 100% (2/2) | 30 | 5 | 25 | 83 |