Table 5. Co-expression of Orco and Ir25a in non-melanogaster insect olfactory organs (related to Figure 7).

Whole-mount palps from Drosophila sechellia flies, and whole-mount antennae and palps from Anopheles coluzzii mosquitoes, were examined using fluorescence in situ hybridization with probe sets against Orco and Ir25a. Co-expression between Orco and Ir25a co-receptors was observed in both insects, with D. sec. palps having the highest degree of co-expression (63% of cells double labeled) and A. col. antennae having the lowest (25% of cells double labeled). N = 5 for D. sec. and 7 for A. col.

Species	Sample	Orco+ cells	Ir25a+ cells	Double-labeled cells	Total cells
Drosophila sechellia	Palp 1	70	44	44	70
Drosophila sechellia	Palp 2	64	48	42	70
Drosophila sechellia	Palp 3	63	39	39	63
Drosophila sechellia	Palp 4	78	38	35	81
Drosophila sechellia	Palp 5	86	75	74	87
	Total across samples:	361	244	234	371
		Proportion of Orco+ cells that are Ir25a+:	Proportion of Ir25a+ cells that are Orco+:	Proportion of all cells that are double labeled:
		0.65	0.96	0.63
Anopheles coluzzii	Antenna 1	52	17	12	57
Anopheles coluzzii	Antenna 2	47	24	17	54
Anopheles coluzzii	Antenna 3	57	20	13	64
Anopheles coluzzii	Antenna 4	50	27	14	63
Anopheles coluzzii	Antenna 5	62	30	18	74
Anopheles coluzzii	Antenna 6	53	21	17	57
Anopheles coluzzii	Antenna 7	49	26	16	59
	Total across samples:	370	165	107	428
		Proportion of Orco+ cells that are Ir25a+:	Proportion of Ir25a+ cells that are Orco+:	Proportion of all cells that are double labeled:
		0.29	0.65	0.25
Anopheles coluzzii	Palp 1	34	30	23	41
Anopheles coluzzii	Palp 2	30	36	22	44
Anopheles coluzzii	Palp 3	26	35	19	42
Anopheles coluzzii	Palp 4	35	34	19	50
Anopheles coluzzii	Palp 5	32	39	22	49
Anopheles coluzzii	Palp 6	31	35	21	45
Anopheles coluzzii	Palp 7	30	37	23	44
	Total across samples:	218	246	149	315
		Proportion of Orco+ cells that are Ir25a+:	Proportion of Ir25a+ cells that are Orco+:	Proportion of all cells that are double labeled:
		0.68	0.61	0.47