Table 4.

WGS data for P. aeruginosa isolates from Kuwait and Oman

Isolates stratified by country	IHMA number	C/T MIC (mg/L)	OprD	PBP3 (ftsI)a	WGS lactamase summary (≥72% identity; ≥80% CDS)b	Class C (intrinsic)	MLSTc
Kuwait
1	1562186	8	Frameshift, 195 AA protein	WT	PDC-109	PDC-109	499
2	1562187	>32	No lesion	WT	PDC-119-like; OXA-4; VIM-2	PDC-119-like	233
3	1562188	>32	No lesion	WT	PDC-252-like; OXA-4; VIM-2	PDC-252-like	233
4	1562192	>32	Q158STOP	WT	PDC-119-like; OXA-4; VIM-2	PDC-119-like	233
5	1562196	>32	Frameshift, 359 AA protein	A539T	PDC-252-like; VIM-2; OXA-4	PDC-252-like	233
6	1562198	>32	No lesion	WT	PDC-35; LCR-1; VIM-2; OXA-2	PDC-35	2613
7	1572968	>32	No lesion	WT	PDC-252-like; VIM-2	PDC-252-like	3482
8	1572981	>32	No lesion	WT	PDC-119-like; VIM-2;	PDC-119-like	233
9	1572982	>32	W138STOP	WT	PDC-119-like; OXA-4-like; VIM-2	PDC-119-like	233
10	1572988	>32	Frameshift, 350 AA protein	WT	PDC-35; OXA-2; VIM-6; OXA-10	PDC-35	2613
11	1607795	8	No lesion	WT	PDC-30	PDC-30	671
12	1607809	>32	Disrupted by insertion	WT	PDC-119-like; OXA-4; VIM-2	PDC-119-like	233
13	1607996	>32	No lesion	WT	PDC-35; LCR-1; VIM-2; OXA-2	PDC-35	2613
14	1652654	>32	Frameshift, 354 AA protein	WT	PDC-252-like; OXA-4; VIM-2	PDC-252-like	233
15	1652655	>32	Disrupted by insertion	WT	PDC-252-like; OXA-4; VIM-2	PDC-252-like	233
16	1652663	>32	Frameshift, 135 AA protein	WT	PDC-11; OXA-10; VEB-1	PDC-11	357
17	1723966	>32	Frameshift, 135 AA protein	WT	PDC-11; OXA-10; VEB-1	PDC-11	357
18	1724010	>32	Frameshift, 135 AA protein	WT	PDC-11; VEB-1; OXA-10	PDC-11	357
19	1724024	>32	No lesion	WT	PDC-35; OXA-2; VIM-2; LCR-1	PDC-35	2613
20	1724321	>32	Frameshift, 135 AA protein	WT	PDC-11; OXA-10; VEB-1	PDC-11	357
21	1724327	>32	Frameshift, 135 AA protein	WT	PDC-11; OXA-10; VEB-1	PDC-11	357
22	1734172	8	E176STOP	WT	PDC-212-like	PDC-212-like	272
23	1734205	>32	Disrupted by insertion	WT	PDC-252-like; OXA-4; VIM-2	PDC-252-like	233
24	1734215	>32	Frameshift, 354 AA protein	WT	PDC-119-like; OXA-4; VIM-2	PDC-119-like	233
25	1734223	16	Q424STOP	WT	PDC-279-like	PDC-279-like	244
Oman
1	1688133	>32	Frameshift, 218 AA protein	L346M; F533L	PDC-98; OXA-10; OXA-1-like	PDC-98	664
2	1688134	>32	No lesion	WT	PDC-30	PDC-30	207

AA, amino acid; C/T, ceftolozane/tazobactam.

^aAmino acid changes are greater relative to reference sequences.

^bThreshold for β-lactamase gene inclusion was 72% and 80% for minimum nucleotide sequence identity and minimum sequence length, respectively.

^cNovel MLSTs were given sequential designations for clarity.