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. 2022 Apr 21;77(3):653–659. doi: 10.1016/j.jhep.2022.03.040

Fig. 3.

Fig. 3

Immunophenotype of peripheral and vaccine-elicited CD8 T cells correlates with hepatitis severity.

(A) t-SNE representation of flow cytometry data comparing non-naïve CD8+ T cells 27 dpb in the patient’s liver (red) and blood (blue). Expression levels of CXCR6, CD69, CD103 and CD38 are indicated (blue: low expression; red: high expression). (B) Quantified expression levels of CD38, 27 dpb on non-naïve CD8+ T cells within the patient’s liver (red) and blood (blue) compared to timepoint matched vaccinees without development of hepatitis. (C) Original bivariate plots showing frequencies of spike-specific (A∗03/S378) CD8+ T cells within the CD8+ T-cell population at 27 dpb, liver (red) and blood (blue). Expression levels of CXCR6, CD69, CD103 and CD38 of spike-specific CD8+ T cells are indicated. (D) Longitudinal analysis of calculated ex vivo spike-specific CD8+ T-cell frequencies. (E) Longitudinal analysis of CD38, (F) GrzB, (G) Tbet+CD38hi CD8+ T cells are shown with time-matched ALT (U/L) levels depicted in grey with connected dots, circulating non-naïve spike-specific CD8+ T cells (blue circle) and non-naïve bulk CD8+ T cells (grey open circle). Colored background indicates the patient’s therapy regime (light blue: budesonide, grey: prednisolone). ALT, alanine aminotransferase; dpb, days post boost; GrzB, granzyme B.