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. 2022 Apr 20;8:46. doi: 10.1038/s41531-022-00311-0

Fig. 5. Cdk5 is involved in the nuclear translocation of SIRT2.

Fig. 5

a Co-Immunoprecipitation (Co-IP) was performed to identify whether endogenous SIRT2 interacts with Cdk5. b A GST-pull-down assay was used to verify whether exogenous SIRT2 can bind to Cdk5. c Cultured primary neurons were pretreated with 10 μM roscovitine (ROS) for 0.5 h and subsequently treated with 50 μM MPP+ for 24 h. The cytoplasmic and nuclear distribution of SIRT2 was detected using immunoblotting. d Quantification of SIRT2 protein levels in the cytoplasm and nucleus shown in c (n = 3). e Immunofluorescence staining for SIRT2 (green) was performed to observe the distribution of SIRT2 in primary culture neurons subjected to ROS stress. The scale bar represents 10 μm. All data are presented as the means ± SD. Statistical analyses were conducted using one-way ANOVA followed by Tukey’s post hoc test in d. *P < 0.05, **P < 0.01, ***P < 0.001.