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. 2022 Apr 20;12:6534. doi: 10.1038/s41598-022-10410-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Establishing P. syringae pathology assays on B. napus and C. sativa. (a) P. syringae pv. tomato strain DC3000 (PtoDC3000) causes disease symptoms on B. napus and C. sativa similar to those on A. thaliana. A. thaliana Col-0, B. napus var. Topas, and C. sativa var. DH55 were spray inoculated with either a mock treatment of 10 mM MgSO₄ 0.04% Silwet (Mock) or PtoDC3000 at an OD₆₀₀ = 1. A. thaliana was sprayed at 4 weeks old. B. napus and C. sativa were sprayed at 2 weeks old. Images show 2 leaves per plant. Photographs were taken at 6 days post-infection. Scale bar = 3 cm. (b) HopBA1a elicits ETI in B. napus and C. sativa. Representative photographs of chlorotic symptoms on A. thaliana Col-0, B. napus var. Topas, and C. sativa var. DH55 infected with PtoDC3000 expressing an empty vector (EV), HopBA1a, HopBA1a H56F, or HopBA1a W112A. Plants were spray inoculated as described above. Images show 2 leaves per plant. Photographs were taken at 6 days post-infection. Scale bar = 3 cm.