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. 2022 Apr 20;13(4):380. doi: 10.1038/s41419-022-04825-6

Fig. 5. Exosomal miR-146a-5p and miR-155-5p promoted activation of CAFs through targeting ZBTB2 and SOCS1.

Fig. 5

A Schematic of wild type (WT) and mutant (MUT) binding sites of miR-155-5p and miR-146a-5p on 3′UTR of target gene SOCS1 and ZBTB2 respectively. B HCT116 cells were transfected with luciferase constructs and miR-146a-5p mimics. The comparison of luciferase activity of wild type (WT) and mutant (MUT)-ZBTB2-3′UTR constructs was performed 36 h after transfection. Data were normalized to renilla activity. **p < 0.01 vs. control group. Control-luc represents control luciferase plasmid, ZBTB-3′UTR-WT(MUT)-luc represents the wild type or mutant ZBTB2-3′UTR luciferase constructs. C RT-qPCR analysis of ZBTB2 and SOCS1 mRNA levels in MRC-5 cells transfected with miR-146a-5p and miR-155-5p mimics (146m, 155m) and inhibitors (146i,155i). **p < 0.01 vs. negative control (NC). D, E Western blot analysis of the expression of JAK2, p-STAT3/STAT3, NF-κB, α-SMA, and FAP in MRC-5 cells transfected with miR-155-5p and miR-146a-5p mimics (155m, 146m) and inhibitors (155i, 146i) or treated with exosomes from HCT116CXCR7 and SW620CXCR7 cells and the corresponding controls. The statistical analysis was performed. *p < 0.05 vs. control, **p < 0.01 vs. control.