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. 2022 Apr 20;12:6522. doi: 10.1038/s41598-022-10522-3

Figure 5.

Figure 5

Gpnmb has a protective effect against vascular pathology. (a,b) Endothelium-dependent (left) and endothelium-independent (right) vasorelaxation were examined in the iliac arteries of Gpnmb knockout (Gpnmb KO) mice and littermate controls (WT) after feeding an HFD for 8 weeks (n = 9 each) (a), or in arteries from Gpnmb-overexpressing transgenic (Gpnmb Tg) mice and littermate controls (WT) after feeding the HFD for 8 weeks (n = 6 each) (b). (c,d) Laser Doppler perfusion imaging of hind limbs from Gpnmb KO mice and littermate controls (WT) (c), or Gpnmb Tg mice and littermate controls (WT), after 14 days of ischemia (d). The right graphs show quantification of blood flow recovery on Laser Doppler perfusion imaging at the indicated times (c, n = 9 each; d, n = 9 for WT and n = 6 for Tg). (e,f) Oil red O staining of thoraco-abdominal aortas from ApoE KO mice and ApoE KO/Gpnmb KO (DKO) mice (e), or ApoE KO mice and ApoE KO/Gpnmb Tg mice (f). The right graphs display quantification of the plaque area in the thoraco-abdominal aorta (e, n = 6 for ApoE KO and n = 4 for ApoE KO/Gpnmb KO; f, n = 6 each). Data were analyzed by repeated measures analysis followed by Tukey’s multiple comparison test (a–d), or by the two-tailed Student’s t test (e and f). *P < 0.05, **P < 0.01, NS (not significant). The data are shown as the mean ± SEM with plots of all individual data (a–d) or box and whisker plots (e,f).