Skip to main content
. 2022 Apr 7;13:874459. doi: 10.3389/fimmu.2022.874459

Figure 4.

Figure 4

TLR4 and NF-κB are essential for silica-induced pyroptosis of RAW-ASC cells. RAW-ASC cells are pre-treated with LPS for 6 h, then exposed to silica for 4 h. (A) Expression of TLR4 in RAW-ASC cells determined by Western Blot. (B) Quantitative analysis and comparison of TLR4 by normalizing to GAPDH between groups. N=3. (C) Images of immunofluorescent staining showing the expression of TLR4 in RAW-ASC cells. TLR4 is stained in red; the nuclei are stained in blue. (D) Quantitative analysis and comparison of TLR4 between groups. N=5. (E) Expression of TLR4, NLRP3, ASC, Pro-caspase-1, P20, Pro-IL-1β, cleaved- IL-1β, and IL-6. RAW-ASC cells are pre-treated with TAK-242 for 1 h, then exposed to LPS for 6 h and silica for 4 h. (F) Quantitative analysis and comparison of target proteins in E by normalizing to GAPDH. N=3. (G) Images of immunofluorescent staining showing the expression of NF-κB in RAW-ASC cells. NF-κB is stained in green; the nuclei are stained in blue. (H) Expression of NF-κB, NLRP3, ASC, Pro-caspase-1, P20, Pro-IL-1β, cleaved- IL-1β, and IL-6. RAW-ASC cells are pre-treated with BAY 11-7082 for 1 h, then exposed to LPS for 6 h and silica for 4 h. (I) Quantitative analysis and comparison of protein in H by normalizing to β-actin or GAPDH. N=3. *P < 0.05; ns, not significant.