Table 1.
62 HBV epitopes and their HLA-A cross-restrictions validated by multiple approaches.
| Epitope name | Protein | Epitope sequence | HBV genotype | Cons (%) | Predicted HLA restriction | ELISPOT positive | Activating CD8+ T cell | Reported HLA restriction | Bioinformatics analysis | Peptide competitive binding assay for HLA-A | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Patient’s HLA | Patient’s HLA | Prime-affinity | High affinity | Inter affinity | Low affinity | No affinity | |||||||
| P2 | HBsAg | FLLTRILTI | C/D | ≥ 95 | A0201, A0206 A0207 | A0201/1136 | A0201, A0205 A0206 (37) | A0201 | A0207 | A0206 | A1101 | ||
| P6 | HBsAg | LLCLIFLLV | A/B/C/D | ≥ 95 | A0201, A0101 | A0201/1136 | A0201/0207A0201/3001 | A02 (38) | A0207 | A1101>A0101>A0201 | |||
| P7 | HBsAg | WLSLLVPFV | A/B/C/D | ≥ 95 | A0201, A0206 A0207, A0203 | A0201/1136 A2402/2402 A1101/1101 | A0201, A0202 A0206, A0203 A0207, A0205 (39) | A0203 >A0201>A0206 | A0207 | A1101>A2402 | |||
| P8 | HBsAg | GLSPTVWLSV | A/B/C/D | ≥ 95 | A0201 | A0201/1136 | A0201/3001 | A0201 (40) | A0201 | A1101 | |||
| P13 | HBsAg | SMYPSCCCTK | D | ≥ 95 | A1101, A1102 A0301 | A0201/0206 | A0201>A1101>A0301 | A1101 >A0301>A1102 | A0206 | ||||
| P14 | HBsAg | ASPISSIFSR | C | 80-95 | A1101 | A0206/1101 | A1101 | A1101 | A0206 | ||||
| P19 | HBsAg | LYSILSPFL | C/D | 80-95 | A2402 | A2402/2402 A0201/1136 A0201/3201 A0201/0206 | A2402 | A2402 | A0206>A0201>A1101 | ||||
| P20 | HBsAg | LFILLLCLI | A/C/D | ≥ 95 | A2402 | A2402/2402 A1101/1101 | A2402 | A1101>A2402 | |||||
| P38 | HBsAg | SPISSIFSR | C | 80-95 | A0201, A1101 | A0201/0201 A1101/3101 | A0201>A1101 | A1101>A3101>A0201 | |||||
| P39 | HBsAg | SAISSISSK | B | < 80 | A1101 | A1101/0203 | A1101>A0203 | A1101 | A0203 | ||||
| P40 | HBsAg | QAGFFSLTK | B | < 80 | A1101, A1102 | A1101/3101 | A1101/1101 | A1101>A3101 | A1101 | A3101 | |||
| P41 | HBsAg | QAGFFLLTR | C/D | ≥ 95 | A1101, A1102 | A1101/0201 | A0201/2402 | A1101>A1102 | A1101>A1102>A0201 | ||||
| P51 | HBsAg | VWLSVIWMMW | A/B/C/D | ≥ 95 | A2402 | A0201/0207 | A1101/1102 | A2402>A0201>A0207 | A2402 | A0207 | A0201 | ||
| P71 | HBsAg | MMWFWGPSL | B | 80-95 | A0207, A0301 A0101 | A2402/2402 A2402/24109 | A2402>A0207>A0301 | A0207>A2402 | A0301 | A0101 | |||
| P72 | HBsAg | MMWYWGPSL | A/C/D | ≥ 95 | A0207, A1102 A0301, A0101 | A0201/0201 A2402/0207 | A0201>A0207>A2402 | A0201>A2402>A0207 | A0301 | A0101>A1101>A1102 | |||
| P77 | HBsAg | CPGYRWMCLR | A/B/C/D | ≥ 95 | A3303 | A0201/0201 | A0201/3303 | A3303 | A3303>A0201 | ||||
| P78 | HBsAg | FLWEWASVR | C | ≥ 95 | A3303 | A0201/2402 | A0201>A3303>A2402 | A0201> A3303 | A2402 | ||||
| P95 | HBsAg | LLDYQGMLPV | A/B/C/D | ≥ 95 | A0203 | A2402/2402 A2402/2402 | A3303/3001 | A02 (38) | A0203>A2402 | A0203 | A2402 | ||
| P103 | HBsAg | LQAGFFSLTK | B | < 80 | A1102 | A2402/0207 | A0207>A2402>A1102 | A1101 | A0207 | A1102>A2402 | |||
| P108 | HBsAg | MMWYWGPSLY | A/C/D | ≥ 95 | A0301, A0101 A1102 | A2402/0207A2402/0207 | A03 (37) | A2402>A0301>A0207 | A0207>A1101>A0301 | A0101>A2402>A1102 | |||
| P1 | HBeAg | FLPSDFFPSV | A/D | ≥ 95 | A0201, A0203 A0207, A0206 | A0201/1136 A2402/3303 A1101/1101 | A0201, A0203 A0206, A0207 (30) | A0201>A0206>A0203 | A0207 | A2402>A3303>A1101 | |||
| P4 | HBeAg | LVSFGVWIR | A/B/C/D | ≥ 95 | A1101, A1102 | A1101/0206 A0201/0206 | A33 (41) | A1101>A3303 | A0206>A0201>A1102 | ||||
| P11 | HBeAg | YLVSFGVWI | A/B/C/D | ≥ 95 | A0201, A0203 | A0201/1136 A2402/1101 A2402/3303 A2402/0203 | A0203/0203A1101/0301 | A0201 (42) | A0203>A0201 | A2402 | A1101> A3303 | ||
| P22 | HBeAg | SYVNTNMGL | D | 80-95 | A2402 | A2402/2402 A0206/1101 | A0201 (32) | A2402 | A1101>A0201> A0206 | ||||
| P23 | HBeAg | EYLVSFGVW | A/B/C/D | ≥ 95 | A2402 | A1101/1101 A0201/3201 A0207/2402 | A2402, A2407 A2301 (43) | A2402 | A0201>A1101 | ||||
| P31 | HBeAg | FLPSDFFPSI | B/C | ≥ 95 | A0201, A0207 A0203 | A0207/2402 A1101/2463 | A1101/1101 | A0201, A0207 (40) | A0207>A0201>A1101 | A0203>A0201>A2402 | A0207 | A1101 | |
| P32 | HBeAg | LLWFHISCL | A/B/C/D | ≥ 95 | A0201 | A0201/2402 | A2402/1101A0207/0207 | A0201 (42) | A2402>A0201 | ||||
| P42 | HBeAg | STLPETTVVR | A/B/C/D | / | A1101, A1102 | A1101/0201 A1101/2463 A0207/3001 | A1101/1102 | A0201, A11 A6801 (37) | A1101>A0207 | A1101 | A0207 | A1102>A0201>A3001 | |
| P52 | HBeAg | WFHISCLTF | A/B/C/D | ≥ 95 | A2402 | A2402/0207 A0201/0207 | A2402>A0207>A0201 | A2402 | A0207 | A0201 | |||
| P53 | HBeAg | SYVNVNMGL | A/B/C | 80-95 | A2402 | A2402/0207 | A0207/0206A0201/2402A0206/1101 | A2402>A0207 | A2402 | A0207 | |||
| P67 | HBeAg | FLPSDFFPS | A/B/C/D | ≥ 95 | A0206, A0201 A0207, A0203 | A2402/2601 A2402/3303 | A3303/3001 | A02 (44) | A0206>A0201>A0203 | A0207 | A2402>A3303 | ||
| P74 | HBeAg | ILCWGELMNL | B/C | ≥ 95 | A0207 | A2402/0207 A2402/3315 | A0207>A2402 | A0201 | A0207 | A2402>A3303 | |||
| P88 | HBeAg | ASRELVVSY | B/C | 80-95 | A3001 | A0201/0201 A0206/0206 A0101/0206 A0101/0206 A0201/2601 | A0201/0206 | A0201>A3001>A0206 | A3001 | A0201 | A0101>A0206 | ||
| P118 | HBeAg | ETVLEYLVSV | C | ≥ 95 | A2601 | A1101/2402 A1101/2402 A0203/3001 A0201/1101 A0201/0207 | A2601>A1101>A0201 | A0207>A3001 | A1101>A0203>A0201>A2402 | ||||
| P66 | HBeAg | MQLFHLCLI | A/B/C/D | / | A0206 | A0201/0201 | A3303/3001 | A0201 (16) | A0201 | A0206 | |||
| P96 | HBeAg | ILSTLPETTV | A/B/C/D | / | A0203 | A0201/0201 | A02 (45) | A0201>A0203 | A0203 | A0201 | |||
| P111 | HBeAg | LLDTASALY | A/B/D | ≥ 95 | A0101, A1102 | A2402/2402 A2402/0207 | A1101/1101 | A0101>A1102 | A0101 | A0207 | A1101>A0201>A2402>A1102 | ||
| P10 | HBpol | FLLSLGIHL | A/B/C/D | ≥ 95 | A0201, A0206 A0207 | A0201/1136 A0201/0201 A0206/0206 A2402/1101 A2402/3303 A1101/1101 | A0201, A0206 A0202 (37) | A0201>A3303 | A0207>A0206 | A2402>A1101 | |||
| P15 | HBpol | KVTKYLPLDK | D | < 80 | A1101 | A0206/1101 | A1101 | A1101 | A0206 | ||||
| P16 | HBpol | PTYKAFLCK | C/D | < 80 | A1101, A1102 | A1101/0206 | A0203/1101 | A1101>A0206 | A1101 | A1102>A0206 | |||
| P24 | HBpol | KYTSFPWLL | A/B/C/D | ≥ 95 | A2402 | A0201/0206 | A2301, A2402 (46) | A2402 | A0201>A0206 | ||||
| P25 | HBpol | FYPKVTKYL | D | < 80 | A2402 | A1101/1101 | A2402>A1101 | A2402 | A1101 | ||||
| P33 | HBpol | KLIGTHNSV | B | < 80 | A0201, A0203 | A0201/0201 A1101/0203 | A0201>A0203>A1101 | A0201 | A0203 | A1101 | |||
| P34 | HBpol | KLIGTDNSV | A/C | 80-95 | A0201, A0203 | A0201/2402 | A1101/1101A3303/2601 | A0201>A0203 | A0201 | A0203 | A2402 | ||
| P55 | HBpol | LYSSTVPCF | B | < 80 | A2402 | A0201/3303 | A0201/2402 | A2402>A3303>A0201 | A2402 | A0201>A3303 | |||
| P56 | HBpol | LYSSTVPVF | C | ≥ 95 | A2402 | A0201/0207 | A2402>A0207 | A2402 | A0207 | A0201 | |||
| P76 | HBpol | FLLAQFTSA | A/B/C/D | ≥ 95 | A0207, A0203 | A0207/1101 | A0203>A0207 | A0207>A0203 | A1101 | ||||
| P89 | HBpol | RSRSGAKLI | B/C | 80-95 | A3001 | A0201/0201 A0101/0206 A0206/0206 | A0201/3001 | A0201>A0206>A3001 | A3001 | A0201 | A0206>A0101 | ||
| P90 | HBpol | RSRSGANIL | C/D | ≥ 95 | A3001 | A0201/0201 | A0201>A3001 | A3001 | A0201 | ||||
| P98 | HBpol | LLAQFTSAI | A/B/C/D | ≥ 95 | A0203 | A0203/0301 A0203/0201 | A0201 (42) | A0203 | A0203 | A0201 | |||
| P106 | HBpol | TLWKAGILYK | A/B/C/D | ≥ 95 | A1102, A0301 | A2402/2402 A2402/2402 A2402/2402 | A03 (47) | A0301>A2402>A1102 | A0301 | A1101 | A1102>A2402 | ||
| P119 | HBpol | HTAELLAACF | A/B/C/D | ≥ 95 | A2601 | A2402/2402 | A2402>A2601 | A2402 | |||||
| P12 | HBx | CLFKDWEEL | D | ≥ 95 | A0201 | A0201/1136 | A0201 (48) | A0201 | A1101 | ||||
| P17 | HBx | TVNAHQILPK | A/D | < 80 | A1101, A1102 A0301 | A1101/3001 | A3303/2601 | A1101>A3001 | A1101 | A3001 | |||
| P18 | HBx | STTDLEAYFK | A/B/C/D | ≥ 95 | A1101 | A0201/0206 A0206/1101 | A0201>A1101>A0206 | A1101 | A0206>A0201 | ||||
| P26 | HBx | VCAPAPCNF | D | 80-95 | A2402 | A0206/1101 A2402/2402 | A1101/3303 | A2402 | A1101> A2402>A0206 | ||||
| P36 | HBx | VLGGCRHKL | A/B/C/D | ≥ 95 | A0201 | A1101/3101 | A0201 (49) | A0201 | A1101>A3101 | ||||
| P47 | HBx | TVNAHGNLPK | B | < 80 | A1101, A1102 A0301 | A1101/0201 | A0201>A1101>A0301 | A1101>A0301 | A1102 | A0201 | |||
| P48 | HBx | TVNAHQVLPK | C | < 80 | A1101, A1102 A0301 | A1101/0201 | A1101/1102 | A1101 | A1101>A0301 | A1102 | A0201 | ||
| P63 | HBx | KVFVLGGCR | A/B/C/D | < 80 | A3101, A0301 | A2402/2402 | A1101/1101A3303/2601 | A2402>A3101 | A3101 | A2402 | |||
| P93 | HBx | RLKVFVLGG | A/B/C/D | < 80 | A3001 | A2402/2402 | A2402>A3001 | A3001>A2402 | |||||
| P100 | HBx | HLSLRGLPV | A/B/C/D | ≥ 95 | A0203 | A2402/2402 A2402/2402 A2402/2402 | A1101/1101 | A0201 (49) | A0203 | A2402 | |||
ELISPOT positive, the epitope peptide induced positive T cell response in the ex vivo IFN-γ ELISPOT assay with the indicated patient’s PBMCs. Activating CD8+ T cell, the epitope peptide induced positive CD8+ T cell response in the in vitro cocultures with the indicated patient’ PBMCs. Cons (%), conservation (%).