Table 2.
Binding affinity of 62 HBV T cell epitopes with twelve prevalent HLA-A allotypes as detected by peptide competitive binding experiments using transfected Hmy2. CIR cell lines.
| HLA-A | Peptides | Affinity | 5μM Inhibition (%) | 15μM Inhibition (%) | Sequence |
|---|---|---|---|---|---|
| A0201 | P1 | high | 94.60% | 98.20% | FLPSDFFPSV |
| P67 | high | 91.53% | 97.83% | FLPSDFFPS | |
| P72 | high | 88.70% | 93.40% | MMWYWGPSL | |
| P7 | high | 83.00% | 96.40% | WLSLLVPFV | |
| P10 | high | 81.70% | 71.60% | FLLSLGIHL | |
| P11 | high | 81.20% | 96.20% | YLVSFGVWI | |
| P2 | high | 80.00% | 87.80% | FLLTRILTI | |
| P78 | high | 79.72% | 97.30% | FLWEWASVR | |
| P31 | high | 79.00% | 90.40% | FLPSDFFPSI | |
| P66 | high | 70.10% | 83.50% | MQLFHLCLI | |
| P12 | high | 64.30% | 80.40% | CLFKDWEEL | |
| P34 | high | 50.00% | 52.30% | KLIGTDNSV | |
| P88 | inter | 47.90% | 58.60% | ASRELVVSY | |
| P33 | inter | 37.90% | 50.00% | KLIGTHNSV | |
| P74 | inter | 33.15% | 74.09% | ILCWGELMNL | |
| P96 | inter | 20.50% | 51.00% | ILSTLPETTV | |
| P89 | low | 25.80% | 27.10% | RSRSGAKLI | |
| P36 | low | 22.30% | 37.30% | VLGGCRHKL | |
| P90 | low | 19.60% | 28.70% | RSRSGANIL | |
| P98 | low | 15.26% | 32.81% | LLAQFTSAI | |
| P8 | low | 6.70% | 30.50% | GLSPTVWLSV | |
| P24 | no | 13.00% | 12.30% | KYTSFPWLL | |
| P22 | no | 11.30% | 11.00% | SYVNTNMGL | |
| P23 | no | 10.76% | 8.18% | EYLVSFGVW | |
| P111 | no | 10.60% | 14.60% | LLDTASALY | |
| P48 | no | 9.50% | 15.00% | TVNAHQVLPK | |
| P18 | no | 9.40% | 10.00% | STTDLEAYFK | |
| P55 | no | 8.20% | 11.60% | LYSSTVPCF | |
| P118 | no | 7.95% | 11.78% | ETVLEYLVSV | |
| P4 | no | 7.90% | 11.90% | LVSFGVWIR | |
| P52 | no | 7.39% | 7.05% | WFHISCLTF | |
| P47 | no | 7.20% | 12.00% | TVNAHGNLPK | |
| P42 | no | 6.20% | 14.20% | STLPETTVVR | |
| P56 | no | 5.48% | 5.03% | LYSSTVPVF | |
| P41 | no | 5.00% | 11.40% | QAGFFLLTR | |
| P19 | no | 3.60% | 13.10% | LYSILSPFL | |
| P51 | no | 2.00% | 4.50% | VWLSVIWMMW | |
| P6 | no | 0.00% | 14.80% | LLCLIFLLV | |
| P32 | no | 0.00% | 0.00% | LLWFHISCL | |
| P38 | no | 0.00% | 0.00% | SPISSIFSR | |
| P77 | no | 0.00% | 0.00% | CPGYRWMCLR | |
| A1101 | P103 | high | 89.10% | 89.40% | LQAGFFSLTK |
| P40 | high | 87.66% | 92.84% | QAGFFSLTK | |
| P48 | high | 86.00% | 90.95% | TVNAHQVLPK | |
| P39 | high | 85.95% | 92.42% | SAISSISSK | |
| P13 | high | 79.48% | 83.91% | SMYPSCCCTK | |
| P47 | high | 78.34% | 90.30% | TVNAHGNLPK | |
| P17 | high | 65.39% | 84.36% | TVNAHQILPK | |
| P16 | high | 61.41% | 79.25% | PTYKAFLCK | |
| P15 | high | 60.16% | 78.80% | KVTKYLPLDK | |
| P42 | high | 53.80% | 76.98% | STLPETTVVR | |
| P106 | inter | 20.00% | 91.30% | TLWKAGILYK | |
| P14 | low | 36.18% | 45.27% | ASPLSSIFSR | |
| P108 | low | 31.80% | 32.00% | MMWYWGPSLY | |
| P4 | low | 30.05% | 35.95% | LVSFGVWIR | |
| P18 | low | 23.68% | 41.30% | STTDLEAYFK | |
| P11 | no | 17.10% | 20.00% | YLVSFGVWI | |
| P26 | no | 15.30% | 22.40% | VCAPAPCNF | |
| P12 | no | 14.90% | 22.60% | CLFKDWEEL | |
| P72 | no | 14.30% | 14.70% | MMWYWGPSL | |
| P8 | no | 14.10% | 11.50% | GLSPTVWLSV | |
| P36 | no | 14.00% | 20.60% | VLGGCRHKL | |
| P6 | no | 13.50% | 10.00% | LLCLIFLLV | |
| P118 | no | 13.40% | 14.60% | ETVLEYLVSV | |
| P38 | no | 13.23% | 14.59% | SPISSIFSR | |
| P22 | no | 12.00% | 16.20% | SYVNTNMGL | |
| P41 | no | 11.98% | 12.89% | QAGFFLLTR | |
| P111 | no | 11.00% | 14.80% | LLDTASALY | |
| P23 | no | 8.91% | 1.64% | EYLVSFGVW | |
| P31 | no | 8.50% | 9.50% | FLPSDFFPSI | |
| P33 | no | 8.00% | 14.80% | KLIGTHNSV | |
| P10 | no | 7.20% | 8.00% | FLLSLGIHL | |
| P7 | no | 5.50% | 3.11% | WLSLLVPFV | |
| P20 | no | 5.05% | 4.48% | IFILLLCLI | |
| P2 | no | 4.50% | 8.50% | FLLTRILTI | |
| P25 | no | 4.14% | 9.48% | FYPKVTKYL | |
| P1 | no | 1.41% | 5.95% | FLPSDFFPSV | |
| P19 | no | 0.90% | 16.20% | LYSILSPFL | |
| P76 | no | 0.00% | 0.00% | FLLAQFTSA | |
| A3303 | P78 | high | 72.10% | 89.60% | FLWEWASVR |
| P10 | high | 60.14% | 66.07% | FLLSLGIHL | |
| P4 | low | 27.37% | 33.02% | LVSFGVWIR | |
| P11 | no | 10.25% | 4.63% | YLVSFGVWI | |
| P1 | no | 7.10% | 10.02% | FLPSDFFPSV | |
| P77 | no | 6.50% | 10.70% | CPGYRWMCLR | |
| P55 | no | 0.00% | 0.00% | LYSSTVPCF | |
| P67 | no | 0.00% | 0.00% | FLPSDFFPS | |
| P74 | no | 0.00% | 0.00% | ILCWGELMNL | |
| A0203 | P96 | high | 100.00% | 99.40% | ILSTLPETTV |
| P95 | high | 97.70% | 95.95% | LLDYQGMLPV | |
| P7 | high | 96.30% | 98.70% | WLSLLVPFV | |
| P11 | high | 89.40% | 97.40% | YLVSFGVWI | |
| P100 | high | 88.60% | 98.90% | HLSLRGLPV | |
| P1 | high | 87.34% | 92.11% | FLPSDFFPSV | |
| P31 | high | 86.26% | 90.92% | FLPSDFFPSI | |
| P67 | high | 86.26% | 91.52% | FLPSDFFPS | |
| P98 | high | 52.37% | 82.68% | LLAQFTSAI | |
| P34 | inter | 41.52% | 69.32% | KLIGTDNSV | |
| P33 | low | 20.63% | 41.87% | KLIGTHNSV | |
| P76 | low | 20.39% | 36.86% | FLLAQFTSA | |
| P118 | no | 11.92% | 17.05% | ETVLEYLVSV | |
| P39 | no | 2.61% | 3.81% | SAISSISSK | |
| A0206 | P67 | high | 95.70% | 100.00% | FLPSDFFPS |
| P1 | high | 94.39% | 100.91% | FLPSDFFPSV | |
| P7 | high | 82.90% | 96.20% | WLSLLVPFV | |
| P66 | inter | 27.30% | 51.60% | MQLFHLCLI | |
| P2 | low | 22.43% | 39.90% | FLLTRILTI | |
| P10 | low | 21.50% | 43.20% | FLLSLGIHL | |
| P89 | no | 15.60% | 12.10% | RSRSGAKLI | |
| P4 | no | 15.12% | 14.21% | LVSFGVWIR | |
| P19 | no | 14.86% | 10.56% | LYSILSPFL | |
| P13 | no | 12.13% | 10.17% | SMYPSCCCTK | |
| P18 | no | 10.82% | 13.43% | STTDLEAYFK | |
| P15 | no | 10.56% | 9.91% | KVTKYLPLDK | |
| P24 | no | 9.39% | 9.13% | KYTSFPWLL | |
| P16 | no | 9.00% | 8.08% | PTYKAFLCK | |
| P26 | no | 8.08% | 7.69% | VCAPAPCNF | |
| P22 | no | 7.95% | 11.21% | SYVNTNMGL | |
| P14 | no | 7.17% | 8.87% | ASPISSIFSR | |
| P88 | no | 0.00% | 0.60% | ASRELVVSY | |
| A2402 | P24 | high | 89.50% | 95.30% | KYTSFPWLL |
| P56 | high | 85.32% | 87.32% | LYSSTVPVF | |
| P25 | high | 82.50% | 83.60% | FYPKVTKYL | |
| P19 | high | 82.00% | 85.00% | LYSILSPFL | |
| P55 | high | 78.21% | 90.82% | LYSSTVPCF | |
| P23 | high | 76.15% | 85.91% | EYLVSFGVW | |
| P22 | high | 72.20% | 85.00% | SYVNTNMGL | |
| P31 | high | 70.60% | 86.10% | FLPSDFFPSI | |
| P53 | high | 68.50% | 74.68% | SYVNVNMGL | |
| P52 | high | 58.21% | 75.85% | WFHISCLTF | |
| P72 | high | 55.70% | 71.40% | MMWYWGPSL | |
| P71 | high | 50.32% | 62.91% | MMWFWGPSL | |
| P11 | inter | 41.00% | 52.50% | YLVSFGVWI | |
| P51 | inter | 39.97% | 52.50% | VWLSVIWMMW | |
| P10 | no | 19.00% | 17.00% | FLLSLGIHL | |
| P34 | no | 11.40% | 12.00% | KLIGTDNSV | |
| P100 | no | 10.26% | 14.68% | HLSLRGLPV | |
| P111 | no | 10.26% | 19.09% | LLDTASALY | |
| P1 | no | 10.00% | 25.00% | FLPSDFFPSV | |
| P63 | no | 9.68% | 4.38% | KVFVLGGCR | |
| P26 | no | 9.68% | 29.09% | VCAPAPCNF | |
| P108 | no | 4.50% | 8.00% | MMWYWGPSLY | |
| P119 | no | 3.10% | 15.85% | HTAELLAACF | |
| P93 | no | 2.62% | 18.79% | RLKVFVLGG | |
| P74 | no | 2.20% | 11.10% | ILCWGELMNL | |
| P106 | no | 2.10% | 16.15% | TLWKAGILYK | |
| P67 | no | 2.00% | 4.30% | FLPSDFFPS | |
| P95 | no | 1.74% | 19.38% | LLDYQGMLPV | |
| P103 | no | 1.60% | 4.50% | LQAGFFSLTK | |
| P78 | no | 1.00% | 9.10% | FLWEWASVR | |
| P20 | no | 0.90% | 1.30% | IFILLLCLI | |
| P32 | no | 0.70% | 0.70% | LLWFHISCL | |
| P118 | no | 0.50% | 3.90% | ETVLEYLVSV | |
| P7 | no | 0.00% | 0.00% | WLSLLVPFV | |
| A3001 | P88 | high | 69.00% | 74.75% | ASRELVVSY |
| P90 | high | 68.00% | 70.00% | RSRSGANIL | |
| P89 | high | 62.25% | 71.75% | RSRSGAKLI | |
| P17 | low | 42.50% | 46.50% | TVNAHQILPK | |
| P118 | low | 24.00% | 26.50% | ETVLEYLVSV | |
| P93 | no | 15.75% | 24.00% | RLKVFVLGG | |
| P42 | no | 4.50% | 4.25% | STLPETTVVR | |
| A0101 | P111 | inter | 31.22% | 62.33% | LLDTASALY |
| P108 | no | 11.22% | 13.78% | MMWYWGPSLY | |
| P89 | no | 10.11% | 9.00% | RSRSGAKLI | |
| P6 | no | 9.44% | 10.11% | LLCLIFLLV | |
| P71 | no | 9.00% | 7.89% | MMWFWGPSL | |
| P72 | no | 9.00% | 9.00% | MMWYWGPSL | |
| P88 | no | 9.00% | 9.00% | ASRELVVSY | |
| A0207 | P71 | high | 52.00% | 59.00% | MMWFWGPSL |
| P72 | high | 52.00% | 54.00% | MMWYWGPSL | |
| P2 | inter | 36.00% | 52.00% | FLLTRILTI | |
| P31 | low | 47.00% | 45.00% | FLPSDFFPSI | |
| P74 | low | 45.00% | 43.00% | ILCWGELMNL | |
| P51 | low | 43.00% | 43.00% | VWLSVIWMMW | |
| P67 | low | 42.00% | 48.00% | FLPSDFFPS | |
| P76 | low | 41.00% | 41.00% | FLLAQFTSA | |
| P118 | low | 40.00% | 39.00% | ETVLEYLVSV | |
| P1 | low | 39.00% | 45.00% | FLPSDFFPSV | |
| P103 | low | 39.00% | 44.00% | LQAGFFSLTK | |
| P7 | low | 38.00% | 25.00% | WLSLLVPFV | |
| P108 | low | 38.00% | 40.00% | MMWYWGPSLY | |
| P111 | low | 37.00% | 39.00% | LLDTASALY | |
| P52 | low | 36.00% | 38.00% | WFHISCLTF | |
| P53 | low | 36.00% | 31.00% | SYVNVNMGL | |
| P42 | low | 33.00% | 45.00% | STLPETTVVR | |
| P56 | low | 32.00% | 36.00% | LYSSTVPVF | |
| P6 | low | 31.00% | 22.00% | LLCLIFLLV | |
| P10 | low | 27.00% | 31.00% | FLLSLGIHL | |
| A3101 | P63 | inter | 35.43% | 55.43% | KVFVLGGCR |
| P40 | no | 4.93% | 5.48% | QAGFFSLTK | |
| P36 | no | 4.82% | 5.70% | VLGGCRHKL | |
| P38 | no | 4.04% | 4.49% | SPISSIFSR | |
| A1102 | P13 | high | 54.67% | 73.07% | SMYPSCCCTK |
| P47 | low | 24.71% | 44.19% | TVNAHGNLPK | |
| P48 | low | 25.52% | 42.71% | TVNAHQVLPK | |
| P103 | no | 18.94% | 25.12% | LQAGFFSLTK | |
| P16 | no | 13.7% | 27.27% | PTYKAFLCK | |
| P42 | no | 12.36% | 25.52% | STLPETTVVR | |
| P106 | no | 8.19% | 8.19% | TLWKAGILYK | |
| P72 | no | 7.12% | 9.54% | MMWYWGPSL | |
| P41 | no | 6.72% | 9.40% | QAGFFLLTR | |
| P111 | no | 0.27% | 0.00% | LLDTASALY | |
| P4 | no | 0.00% | 0.00% | LVSFGVWIR | |
| P108 | no | 0.00% | 0.00% | MMWYWGPSLY | |
| A0301 | P13 | high | 79.85% | 80.7% | SMYPSCCCTK |
| P106 | high | 78.14% | 82.84% | TLWKAGILYK | |
| P48 | high | 67.89% | 73.87% | TVNAHQVLPK | |
| P47 | high | 65.76% | 78.14% | TVNAHGNLPK | |
| P72 | low | 26.9% | 35.87% | MMWYWGPSL | |
| P108 | low | 26.05% | 29.46% | MMWYWGPSLY | |
| P71 | low | 22.2% | 38.00% | MMWFWGPSL |
Binding affinity of epitope peptide with indicated HLA-A allotype was assessed by IC50, which is the concentration of unlabeled competitor peptide required to inhibit the binding of fluoresce-labeled reference peptide by 50%. IC50<5μM (5μM inhibition >50%) means high binding affinity, 5μM<IC50<15μM (5μM inhibition<50%, but 15μM inhibition >50%) means intermediate binding affinity, IC50>15μM means low or no binding affinity. 5μM inhibition 20-50% or 15μM inhibition 30-50% means low binding affinity; 5μM inhibition <20% or 15μM inhibition <30% means no binding affinity.
Most peptides with high or intermediate binding affinity displayed conservative amino acids at P2 position and COOH terminus (bold letter).