Figure 4.

HLA class II restriction results of four representative proinsulin-specific T-cell clones. The restriction of the clones was determined by inhibiting proliferative responses to cognate peptide by anti-HLA-DR, -DQ and -DP antibodies (A), and by using DR3-DQ2 and DR4-DQ8 homozygous EBV-transformed B cell lines (B) or BLS or RM3 cell lines transfected with single HLA class II molecules (C) as APCs. The HLA class II genotype of the subject from which the clone was isolated is indicated below the clone and subject numbers.