Table I.
HLA class II-restriction and core amino acid sequences recognized by the proinsulin-specific T-cell clones.
| Clone | Subject | HLA genotype | Core sequence | Position | Reactivity to | HLA-restriction | ||
|---|---|---|---|---|---|---|---|---|
| C-pep | B9-23 | Proinsulin | ||||||
| 1 | 001 | DR3/DR4 | PLALEGSLQK | C25-34 | No | * | No | DRB1*0404 |
| 2 | 001 | DR3/DR4 | PLALEGSLQ | C25-33 | Yes | * | No | DRB1*0404 |
| 3 | 001 | DR3/DR4 | PLALEGSLQK | C25-34 | Yes | * | No | DRB1*0404 |
| 4 | 002 | DR3/DR4 | GSLQPLALEGSL | C21-32 | Yes | * | No | DRB4*0101 |
| 5 | 003 | DR3/DR4 | PLALEGSLQ | C25-33 | Yes | * | No | DRB1*0404 |
| 6 | 003 | DR3/DR4 | PLALEGSLQKR | C25-35 | No | * | No | DRB1*0301 |
| 7 | 004 | DR3/DR4 | LQPLALEGSLQKR | C23-35 | No | * | No | DRB1*0401/0404 |
| 8 | 005 | DR3/DR3 | YLVCGERGFF | B16-25 | No | * | No | DR3 |
| 9 | 006 | DR3/DR3 | YLVCGERGFF | B16-25 | * | * | No | DR3 |
| 10 | 006 | DR3/DR3 | YLVCGERGFFYT | B16-27 | No | * | * | DR3 |
| 11 | 007 | DR3/DR3 | QPLALEGSLQKR | C24-35 | No | * | No | DRB1*0301 |
| 12 | 008 | DR3/DR4 | RGFFYTPKTRRE | B22-C3 | No | * | No | DRB1*0301 |
| 13 | 009 | DR3/DR4 | SLQPLALEGSL | C22-32 | Yes | * | No | DRB1*0401 |
| 14 | 010 | DR3/DR4 | YLVCGERGF | B16-24 | No | * | No | DR3 |
| 15 | 011 | DR3/DR4 | GSLQPLALEGSL | C21-32 | * | * | No | DR4 |
| 16 | 012 | DR3/DR4 | GIVEQCCTSICSLY | A1-14 | No | * | * | DR4 |
| 17 | 012 | DR3/DR4 | GIVEQCCTSICSLYQ | A1-15 | * | * | * | DR4 |
| 18 | 013 | DQ2/DQ8 | SHLVEALYLV | B9-18 | No | Yes | * | DQ2 |
| 19 | 014 | DQ2/DQ8 | VCGERGFFY | B18-26 | No | * | Yes | DQ2 |
| 20 | 005 | DQ2/DQ2 | PGAGSLQPLAL | C18-28 | Yes | * | * | DQ2 |
| 21 | 015 | DQ2/DQ2 | HLVEALYLVCG | B10-20 | * | Yes | * | DQ2 |
| 22 | 007 | DQ2/DQ2 | SLQPLALEGSLQ | C22-33 | Yes | * | Yes | DQ2 |
| 23 | 016 | DQ8/DQ8 | GGPGAGSLQPL | C16-26 | Yes | * | * | DQ8 |
| 24 | 017 | DQ2/DQ2 | ALYLVCGERG | B14-23 | No | Yes | * | DQ2 |
*
, not tested