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. 2022 Apr 7;13:819516. doi: 10.3389/fphar.2022.819516

TABLE 1.

Intranasal perfusion of USPIO-loaded mouse astrocyte-derived EVs.

Day Intranasal perfusion of ADEVs and MRI scan
Day 0 • MRI pre-scan on 6 mice
• Wait 1 h and intranasal perfusion of 2 × 1012 USPIO-labeled ADEVs in 50 μL PBS
Day 1 • Intranasal perfusion of 2 × 1012 USPIO-labeled ADEVs in 50 μL PBS
• Wait 1 h and MRI scan
• Intranasal perfusion of 2 × 1012 USPIO-labeled ADEVs in 50 μL PBS
Day 2 • Intranasal perfusion of 2 × 1012 USPIO-labeled ADEVs in 50 μL PBS
• Wait 1 h and MRI scan
• Intranasal perfusion of 2 × 1012 USPIO-labeled ADEVs in 50 μL PBS
Day 3 • Intranasal perfusion of 2 × 1012 USPIO-labeled ADEVs in 50 μL PBS
• Wait 1 h and MRI scan
• Intranasal perfusion of 2 × 1012 USPIO-labeled ADEVs in 50 μL PBS.
Day 4 • Intranasal perfusion of 2 × 1012 USPIO-labeled ADEVs in 50 μL PBS
• Wait 1 h and MRI scan