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. Author manuscript; available in PMC: 2022 Apr 21.
Published in final edited form as: Adv Exp Med Biol. 2018;1068:89–102. doi: 10.1007/978-981-13-0502-3_8

Figure 1. Schematic of monoclonal antibody production by hybridoma technology.

Figure 1.

Laboratory mice are immunized with antigen of interest. Spleen cells are isolated and fused with immortalized myeloma cells such as SP1 cell line. Transformed fused cells are selected under HAT media. Antigen-specific B cells are screened using protein analytic methods (ELISA, Western blotting, flow cytometry). Once B cells of interest are identified, serial dilution will be performed to select for single cells which will be clonal expanded. Lastly, antigen-specific B cell clones will be cultured and antibodies will be purified.