FIG. 1.

Comparison of the amino acid sequences of VanD4 and other d-Ala-d-Lac ligases from glycopeptide-resistant enterococci. The conserved residues that form the active site, and those important for ligand binding in the VanA ligase, are shaded. These are Glu-16, Lys-22, Phe-169, Ser-177, His-244, Glu-250, Arg-290, Phe-294, Tyr-315, and Ser-316 (9, 16). Data for comparison are taken from Genbank accession no. AF130997 (VanD1), AF153050 (VanD2), AF175293 (VanD3), M97297 (VanA), and U35369 (VanB1).