TABLE 3.
d,d-Dipeptidase (VanXD4) and d,d-carboxypeptidase (VanYD4) activity in extracts of E. faecium 10/96A
| Concn of vancomycin (μg/ml) | d,d-Dipeptidase activitya (nmol min−1 mg−1) |
d,d-Carboxypeptidase activityb (nmol min−1 mg−1)
|
|
|---|---|---|---|
| Without penicillin G | With 100 μg of penicillin G/ml | ||
| 0 | 1.0 | 60 | 52 |
| 4 | 1.3 | 62 | 54 |
a
Hydrolysis of 10 mM d-Ala-d-Ala measured in the supernatant of osmotically lysed bacteria after centrifugation at 40,000 × g for 20 min.
b
Hydrolysis of 10 mM UDP-MurNAc-pentapeptide measured in the resuspended pellet fraction after centrifugation at 40,000 × g for 20 min.