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. 2022 Apr 15;12:6335. doi: 10.1038/s41598-022-10018-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Batch processing of human primary immune cells for analysis of 53BP1 spot intensity and γ-H2AX nuclear intensity. CD4 + T, CD8 + T, and monocytes of 10 different donors were processed simultaneously, left untreated or treated with 30 µM ETP for 1.5 h and imaged in high-throughput format. 53BP1 sum spot intensity vs. γ-H2AX mean nuclear intensity were blotted for each donor for (a) CD4 + T cells, (b) CD8 + T cells (c) monocytes. N = 663–5693 cells per sample.