Figure 2.

Female STAT5^AKO mice have increased subcutaneous adiposity when fed chow diet. Female STAT5^AKO (AKO) and floxed (FL) littermate control mice were weaned onto regular chow diet (13% kcal from fat). (A) Representative images of inguinal and gonadal white adipose tissue depots (iWAT and gWAT) from five-month-old mice. (B) Weights of white adipose tissue depots (iWAT, gWAT, retroperitoneal - rWAT, mesenteric - mWAT), brown adipose tissue (BAT), gastrocnemius skeletal muscle (Gastroc), and liver collected from 10-week-old mice (n = 11-13). (C) Mice (n = 5-6 per group) were housed at different temperatures beginning at weaning (3 weeks of age). Body composition was measured at 9 weeks of age, adiposity was calculated as fat mass divided by total body weight for each animal. (D) Representative images of H&E-stained inguinal and gonadal white adipose tissue depots (iWAT and gWAT) from five-month-old mice. Quantification of total mean adipocyte area (E) and fat cell size distribution (F) from H&E-stained images shown in D (n = 4 mice per genotype). Significance was determined by t-test and is denoted as *p < 0.05, **p < 0.01, or ****p < 0.0001 for AKO versus FL comparisons. See also Figures S2, S3 .