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. 2022 Apr 7;13:851967. doi: 10.3389/fendo.2022.851967

Table 6.

Diagnostic performance of lncRNA of various potential combinations of lncRNAs in the Control and Patient groups.

Phenotype Control (n=11) All patients (n=59) DM (n=10) NPDR (n=26) PDR (n=23) NPDR+PDR (n=49) (%total; %NPDR/%PDR)
Groups of 4
 ANRIL+/HOTAIR+/HULC+/H19+ 0 0 0 0 0 0
 ANRIL+/HOTAIR+/HULC+/(H19) 1 (9.1%) 20 (33.9%) 3 (30%) 12 (46.2%) 5 (21.7%) 17 (34.7%; 70.6/29.4)
 (ANRIL)/(HOTAIR)/(HULC)/(H19) 8 (72.7%) 17 (28.8%) 3 (30%) 3 (11.5%) 11 (47.8%) 14 (28.6%; 21.4/78.6)
Groups of 4
 MIAT+/WISPER+/ZFAS1+/H19+ 0 0 0 0 0 0
 (MIAT)/WISPER+/ZFAS1+/(H19) 1 (9.1%) 21 (35.6%) 2 (20%) 14 (53.8%) 5 (21.7%) 19 (38.8%; 73.7/26.3)
 (MIAT)/(WISPER)/(ZFAS1)/(H19) 8 (72.7%) 19 (32.2%) 5 (50%) 5 (19.2%) 9 (39.1%) 14 (28.6%; 35.7/64.3)
Groups of 6
 ANRIL+/HOTAIR+/HULC+/WISPER+/ZFAS1+/H19+ 0 0 0 0 0 0
 ANRIL+/HOTAIR+/HULC+/WISPER+/ZFAS1+/(H19) 1 (9.1%) 18 (30.5%) 3 (30%) 10 (38.5%) 5 (21.7%) 15 (30.6%; 66.7/33.3)
 (ANRIL)/(HOTAIR)/(HULC)/(WISPER)/(ZFAS1)/(H19) 8 (72.7%) 14 (23.7%) 3 (30%) 2 (7.7%) 9 (39.1%) 11 (22.4%; 18.2/81.8)

+ means at least 1SD higher fold of lncRNA expression compared to control’s mean. lncRNA in parenthesis indicate level of lncRNA could not fall below or rise above 1SD of control’s mean. Abbreviations: DR, diabetic retinopathy; DM, diabetes mellitus; PDR, proliferative diabetic retinopathy; NPDR, nonproliferative diabetic retinopathy.

Method:

1. Mean and SD of the control (n=11) was calculated for each of the nine lncRNA.

2. A Z-score was given to each sample based on their distance from the mean and SD of the control.

3. The scores were grouped intro +1SD, +2SD +3SD or more, and -1SD.

4. Phenotypes were formed. ANRIL+ means if ANRIL is expressed at least 1SD above the control’s mean calculated in step 1. (ANRIL) means the level of ANRIL could not fall or rise above 1SD of control’s mean: -1SD < ANRIL < +1SD.

5. The date is shown in this format: #count (%percent) unless the value is 0.

6. Only column NPDR+PDR contains extra information for the percentage of each NPDR and PDR showing the phenotype separately. The first value shows % of NPDR (% of NPDR, % of PDR).