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Increased tissue factor mRNA levels by rh-S-protein in endothelial cell, but not in trophoblast cell cultures. qPCR analysis measured tissue factor (F3) mRNA expression in trophoblast cell lines HTR8/SVNeo (A) and JEG3 (B) as well as endothelial PMVEC (C) and HEEC (D) cultures treated with mock-treated control or 10, 100, or 1000 ng/ml rh-S-protein. Bars represent mean ± SEM, n=3; * P<0.05 vs. control, 10 or 100 ng/ml rh-S-protein in PMVECs; and * P<0.05 vs. control in HEECs.
