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. 2022 Apr 7;13:876555. doi: 10.3389/fimmu.2022.876555

Figure 5.

Figure 5

IFNγ with or without S-protein significantly increases expression of pro-inflammatory cytokines in trophoblastic and endothelial cell cultures. Expression of pro-inflammatory cytokines genes IL-1β, IL-6, IL-8 was measured in HTR8/SVneo (A), JEG3 (B), PMVECs (C), and HEECs (D) treated with either control or 10 ng/ml rh-IFNγ ± 10 ng/ml rh-S-protein by qPCR. Bars represent mean ± SEM, n=3; * P<0.05 vs. control and + P<0.05 vs. rh-IFNγ alone. ELISA results revealed to measure secreted IL-6 and IL-8 protein levels in HTR8 (E) and PMVECs (F) by treatment with 10 ng/ml rh-IFNγ or rh-IFNγ + 10 ng/ml rh-S-protein. Bars represent mean ± SEM, n=3; *P<0.05 vs. control. + P<0.05 vs. IFNγ alone. C, Control; I, rh-IFNγ; and I+S, rh-IFNγ + rh-S-protein.