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. 2022 Apr 7;13:876555. doi: 10.3389/fimmu.2022.876555

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Copyright © 2022 Guo, Semerci, De Assis, Kayisli, Schatz, Steffensen, Guzeloglu-Kayisli and Lockwood

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Recombinant IFNγ with or without S-protein significantly increases mRNA levels of chemokines in trophoblastic and endothelial cell cultures. Expression of chemokines CXCL9, CXCL10 (A); CCL2 and CCL5 (B) in HTR8/SV^neo, expression of chemokines CCL2 and CCL5 (C) in JEG3; expression of chemokines CXCL9, CXCL10 (D), CCL2 and CCL5 (E) in PMVECs; expression of chemokines CXCL9, CXCL10 (F), CCL2 and CCL5 (G) in HEECs treated with either control or 10 ng/ml rh-IFNγ ± 10 ng/ml rh-S-protein treated cells. Bars represent mean ± SEM, n=3; *P<0.05 vs. control. + P<0.05 vs. IFNγ alone. C, Control; I, rh-IFNγ; and I+S, rh-IFNγ + rh-S-protein.