FIGURE 1.

Generation of PfMaCFET-GFP parasites. (A) Schematic for PfMaCFET domains showing an RNA recognition motif (RRM) domain. (B) Schematic for endogenous tagging of the PfMaCFET locus with GFP. The pFCL3_MaCFET_GFP plasmid contains homology arms from the 5′ (5′HR) and 3′ (3′HR) regions of the PfMaCFET locus, a single guide RNA seq (sgRNA), Cas9 and human dihydrofolate reductase (hDHFR). (C) Confirmation of PfMaCFET-GFP parasite generation by genotyping PCR. The oligonucleotides were designed and positions are indicated by arrows in (B) to confirm the introduction of the GFP tag. (D) The expected amplicon sizes for different sets of PCR primer combinations are indicated.