FIGURE 3.

Generation of Pfmacfet¯ parasites. (A) For generation of Pfmacfet¯ parasites a similar strategy as described in Figure 1 was used. pFCL3_PfMaCFET_KO plasmids had homology regions from 5′ (5′HR) and 3′ (3′HR) of the PfMaCFET locus, a single guide RNA seq (sgRNA) and human dihydrofolate reductase (hDHFR). (B,C) PfMaCFET deletion confirmation via diagnostic PCR. The oligonucleotides were designed from outside the 5′HR and 3′HR and the PfMaCFET locus and positions are indicated by arrows in (A). The expected amplicon sizes for different set of PCR primer combinations are indicated in (B,C).