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. 2022 Apr 4;10:825247. doi: 10.3389/fcell.2022.825247

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Copyright © 2022 Kumar, Abatiyow, Haile, Oualim, Leeb, Vaughan and Kappe.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pfmacfet¯ parasites grow normally as asexual parasites and undergo gametocytogenesis. (A) Pfmacfet¯ (clone 2D and 3H) parasites grew at a similar rate as WT PfNF54. Data were averaged from three biological replicates and presented as the mean ± standard deviation (SD). (B) Bar graph shows gametocytemia for WT PfNF54 and Pfmacfet¯ parasites (clone 2D and 3H) measured on day 15. Data were averaged from three biological replicates and presented as the mean ± standard deviation (SD). (C) IFAs performed on thin blood culture smears for WT PfNF54 or Pfmacfet¯ (clone 3H) using α-tubulin (green), a male-specific marker, and Pfg377 (green), a marker for female gametocytes. Representative images are shown. DIC, differential interference contrast. DAPI, 4′,6-diamidino-2-phenylindole, Scale bar = 5 μm.