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. 2022 Apr 4;10:825247. doi: 10.3389/fcell.2022.825247

FIGURE 6.

FIGURE 6

PfMaCFET regulates fertility of female gametes. (A) Mosquitoes with single parasite lines or genetic crosses were dissected on day 7 post feed and number of oocysts were measured per midgut. Data were averaged from three biological replicates with a minimum of 50 mosquito guts and presented as the mean ± standard deviation (SD) n = 3. ****p-value= <0.0001. (B) Oocyst numbers for WT PfNF54 and the WT Pf NF54 × Pfmacfet¯ cross. Error bar indicates mean ± SD; n = 2. NS-Not significant. (C) Confirmation of transmission of Pfmacfet¯ parasites in a WT × Pfmacfet¯ clone 3H genetic cross by genotyping PCR. The oligonucleotides were designed from outside the 5′ and 3′ homology regions of the PfMaCFET locus and positions as indicated by arrows in Figure 3A. (D) Oocyst formation for WT PfNF54, Pfmacfet¯, Pfcdpk4¯ single parasite infections and the Pfmacfet¯ × Pfcdpk4¯ genetic cross infection. Error bar indicates mean ± SD; n = 2. ND-Not detected, **p-value = 0.0048, *p-value = 0.0399.