TABLE 1.

Oligonucleotides used in the study.

Oligonucleotides used for generation of Pf macfet¯ parasites
Oligo	Forward (5′-3′)
PfMaCFET 5′Homo For	TGC​GGC​CGC​GTT​GAC​TAA​ATA​ATT​TTG​AGG​TAT​TCC​ATT​AG
PfMaCFET 5′Homo Rev	CCA​ACC​CGG​GTA​TAG​GCG​CGC​CTG​GAA​GAG​GAA​AAA​TAA​ATG​TAT​ATA​CGG​C
PfMaCFET 3′Homo For	AGG​CGC​GCC​TAT​ACC​CGG​GTT​GGT​ATG​TAT​ATT​ATA​GCA​CAT​GGT​GCT​TCC​C
PfMaCFET 3′Homo Rev	TAA​GTC​GAC​GTA​TTA​TTT​CAC​TTG​ACG​TTT​TTT​TAG​ACC
PfMaCFET Guide 1 For	TAT​TAA​TGT​TGA​CTG​CAA​TAA​AGA
PfMaCFETGuide 1 Rev	AAA​CTC​TTT​ATT​GCA​GTC​AAC​ATT
PfMaCFETGuide 2 For	TAT​TAA​AGA​CAC​ATC​AAA​GCG​TTA
PfMaCFETGuide 2 Rev	AAA​CTA​ACG​CTT​TGA​TGT​GTC​TTT
PfMaCFET Geno5 For	CTA​ATT​TTT​TCT​TTG​GAA​TAA​ATT​TTT​ATG​C
PfMaCFET Geno5 Rev	CAT​TTG​TAA​TGT​ATG​CCA​TTT​CAT​CG
PfMaCFET Geno3 For	AAT​TGG​ACG​CAA​TCG​AGC​AGG
PfMaCFET Geno3 Rev	ACA​CAA​AAA​AGT​GAA​ATG​CTA​AAT​ATT​ATA​CT
Oligonucleotides used for generation of PfMaCFET-GFP parasites
PfMaCFET 5GBlock GFP For 2	ATA​CTA​GTA​TAG​CTA​GCT​ATG​GAA​AGC​ATG​TAA​ATA​TTG​ATA​ATA​TTA​TGA
PfMaCFET 5GBlock GFP Rev 2	AGT​TCT​TCT​CCT​TTA​CTC​ATT​TTT​TGA​ACA​TCA​TAA​GAT​TTG​ACT​ATC​TTA​ATT​TTT​CTT​ATA​TTC​CAT​AAT​GCT​TTT​ATA​TGA​CGC​TCT​AAA​TCC​TGC​TCG​ATT​GCG​TC
PfMaCFET 3G Block GFP_For	GAA​CTA​TAC​AAA​GGG​TAA​GCG​GCC​GCT​TAA​TTC​AAA​AAA​AAC​ATA​TAT​ATA​TAT​ATA​TAT​GTA​TAT​TAT​AG
PfMaCFET 3G Block GFP Rev	CGG​TCA​TGA​ATT​CCT​CGA​GCG​GCC​GCG​TAT​TAT​TTC​ACT​TGA​CGT​TTT​TTT​AG
PfMaCFET Guide5 For	TAT​TAA​AGA​CAC​ATC​AAA​GCG​TTA
PfMaCFET Guide5 Rev	AAA​CTA​ACG​CTT​TGA​TGT​GTC​TTT
GFP For	ATCGTCGACATGAGTAAAGGAGAAGAACTTTTCACTGG