Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 7;13:847008. doi: 10.3389/fimmu.2022.847008

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Soldierer, Bister, Haist, Thivakaran, Cengiz, Sendker, Bartels, Thomitzek, Smorra, Hejazi, Uhrberg, Scheckenbach, Monzel, Wiek, Reinhardt, Niktoreh and Hanenberg

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

CAR NK cells rely on endogenous IL15 production for leukemia control in an ALL xenograft model. (A) Schematic representation of vector maps of CAR constructs used for NK cell transduction. (B) Primary human NK cell preparations consisting of 50% BFP/CD19 CAR, IL15/CD19 CAR, or IL15-IL15R/CD19 CAR and 50% non-transduced NK cells were injected 2 days after xenotransplantation of REH^LucEG into busulfan-preconditioned NSG mice (6 animals per group, 7 animals as untreated control). (C) On days 7, 15, 22, and 27, leukemia progression was analyzed by luminescence imaging in a Caliper device. (D) Kaplan–Meier survival curves for the three treatment groups and the untreated control animals. (E) REH^LucEG and (F) NK cell persistence in the bloodstream was analyzed by CD19, CD34, CD45, and CD56 staining as well as EGFP expression by flow cytometry. Data are represented as mean ± SEM. Significances of p<0.05 are indicated by an asterisk (*).