TABLE 1.
Bacterial strains, plasmids, and primers used in this study
| Strain, plasmid, or primer identifier | Name | Description | Reference or source |
|---|---|---|---|
| Strains | |||
| VJT15.77 | AH-LAC (WT) | ErmS USA300 parent strain | 62 |
| VJT23.52 | ΔlukAB | AH-LAC with a clean deletion of the lukAB operon | 18 |
| VJT58.79 | Δtoxins | AH-LAC mutant strain lacking all bicomponent leukocidins and α-toxin | 71 |
| VJT80.33 | Δatl | AH-LAC carrying the tetM cassette in replacement of the atl gene | This study |
| VJT80.97 | ΔlukAB Δatl | AH-LAC ΔlukAB carrying the tetM cassette in replacement of the atl gene | This study |
| Plasmids | |||
| pOS1-PlukAB-gfp | lukAB promoter controlling expression of a superfolder GFP | This lab | |
| pOS1-Ppvl-gfp | lukSF-pv promoter controlling expression of a superfolder GFP | This lab | |
| pOS1-Psae-gfp | sae P1 promoter controlling expression of a superfolder GFP | 72 | |
| pDB59 PagrP3-yfp | rnaIII promoter controlling expression of YFP | 73 | |
| pJB128 (e.v.) | Insertless complement plasmid control (e.v.) | 33 | |
| pJB111 (AM) | Complement plasmid expressing the amidase domain of Atl (AM) | 33 | |
| pJB135 (GL) | Complement plasmid expressing the glucosaminidase domain of Atl (GL) | 33 | |
| pJB141 (Atl) | Complement plasmid expressing the full-length Atl (Atl) | 33 | |
| Primers | |||
| VJT1836 | tetM.F | GATTGTAAAATAACAAATATTGGTACATG | This study |
| VJT1837 | tetM.R | CAAAAGGTATCAATGAAGCAAGAAATATTG | This study |
| VJT3023 | atl-up.F | CTCTAGAACTAGTGGATCCCCCGGGattagctataaagatgattcacaac | This study |
| VJT3024 | atl-up.R | TACCAATATTTGTTATTTTACAATCtctatttattactcctaacatttattaattattac | This study |
| VJT3025 | atl-down.F | TTTCTTGCTTCATTGATACCTTTTGgcaacatgaacataggatcaaaag | This study |
| VJT3026 | atl-down.R | GCTGGGTACCGGGCCCCCCCTCGAGaataatctctctcttttaatgaagtc | This study |