. 2022 Mar 21;42(4):e00560-21. doi: 10.1128/mcb.00560-21

TABLE 2.

The seven nonsynonymous and intergenic SNPs introduced during whole-genome transformation in the ER18A host strain

SNP no.	Chromosome	Nucleotide in strain			Gene	Nucleotide change in ORF	Amino acid change in protein	No. present in other S. cerevisiae strains
SNP no.	Chromosome	ER18A parent	MS164 transformant	K11 gDNA donor	Gene	Nucleotide change in ORF	Amino acid change in protein	No. present in other S. cerevisiae strains
1	VII	G	T	G	SNF4	c.[805G→T]	Snf4^E269*	0/1,011
2	XIV	C	A	G	Intergenic region	[579C→A] downstream from start codon of FKH2 on Watson strand		0/1,011
3	IV	C	T	C	SEC31	c.[2267C→T]	Sec31^A756V	7/1,011
4	IV	C	A	C	PPN1	c.[227C→A]	Ppn1^P76H	0/1,011
5	X	G	A	G	YJR120W	c.[46G→A]	Yjr120w^A16T	0/1,011
6	XI	C	T	C	TCD2	c.[1051C→T]	Tcd2^P351S	0/1,011
7	XIII	G	A	G	STB4	c.[2285G→A]	Stb4^C762Y	0/1,011