TABLE 3.
Number of WG transformants obtained under selective conditions and number of stable WG transformants obtained after subculturing under nonselective conditions
| Source of gDNA | Selection condition | No. of WG transformants on selective medium | No. of stable WG transformants after subculturing on nonselective mediuma |
|---|---|---|---|
| No DNA (only H2O) | 8 g/L acetic acid | 53 | 0/20 |
| gDNA from non-acetic acid-tolerant strain YPH499 | 8 g/L acetic acid | 243 | 0/20 |
| gDNA from non-acetic acid-tolerant recipient strain ER-18A | 8 g/L acetic acid | 91 | 1/20 |
| Linear fragment with HAA1 | 8 g/L acetic acid | 54 | 4/20 |
| Linear fragment with HAA1* | 8 g/L acetic acid | 96 | 0/20 |
| gDNA of K11-NAT | Nourseothricin | 7 | 0/7 |
| 8 g/L acetic acid | 186 | 2/20 | |
| 8 g/L acetic acid + nourseothricin | 0 | NA | |
| gDNA of 16D-NAT | Nourseothricin | 4 | NT |
| 8 g/L acetic acid | 72 | 2/20 | |
| 8 g/L acetic acid + nourseothricin | 0 | NA | |
a
NA, not applicable; NT, not tested.