TABLE 1.
Ability of dGTP to inhibit primer rescue and to induce DEC formation
| Primer-template | Inhibition of primer rescue (IC50, μM)a ± AD
|
DEC formation (Kd,app, μM)b ± AD | |
|---|---|---|---|
| ATP dependent | PPi dependent | ||
| d4TMP terminated | 0.51 ± 0.07 | 0.72 ± 0.05 | 2.6 ± 1.1 |
| ddTMP terminated | 2.6 ± 0.2 | 1.6 ± 0.5 | 7.7 ± 3.8 |
| AZTMP terminated | 110 ± 30 | 170 ± 20 | 220 ± 60 |
a
Reactions were performed as described in the legend of Fig. 2 in the presence of 67/70/215/219 mutant RT, primer-template terminated with the indicated nucleotide analogue and various concentrations of dGTP. The IC50s were determined as shown in Fig. 2A and B.
b
Electrophoretic mobility shift experiments were performed as described in the text. Kd,app values for DEC formation as a function of dGTP concentration were determined as shown in Fig. 2C. The numbers were obtained from two to three experiments. AD, average deviation.