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. 2022 Apr 7;13:861681. doi: 10.3389/fimmu.2022.861681

Figure 2.

Figure 2

NK cells maintain the expression of activating receptors after cryopreservation. PM21-NK cells expanded from T-cell-depleted PBMCs were obtained from multiple donors (N = 3–7). Cells were cryopreserved while donor-matched fresh PM21-NK cells (black squares) were maintained in culture. Frozen PM21-NK cells were thawed and rested 16 h the day before analysis (blue triangles) or thawed and rested for 1 h on the day of analysis (gray circles). Expression of NK cell activating receptors CD16, NKG2D, DNAM-1, NKp30, NKp46, NKp44, as well as CD69, granzyme B, and perforin was determined by flow cytometry. No statistically significant difference was seen in the expression of activating receptors either 1 h immediately post-thaw or after a 16-h rest in donor and expansion-matched PM21-NK cells (N = 5–7 donors). Data are presented as a scatter plot with each point representing the average of two technical replicates with donor-pair lines. No statistical significance was determined by multiple paired t-tests with a threshold for significance at p < 0.05.