Table 1:

Clinical relevance of TME characterization of neuroblastoma based on RNA-seq.

Cluster	Risk/Outcome	TME Characterization	MYCN Amplification	Pro-Tumor TME Elements	Anti-Tumor TME Elements
1	Ultra-high-risk/Very Poor	Cold, immune excluded	MYCN-A >>> MYCN-NA	Low MHC	Low infiltrate
2	Low-risk/Favorable	Immune irrelevant (Stage 4S)	MYCN-NA	N/A	N/A
3	High-risk/Moderate	Hot, cytotoxic	MYCN-NA >> MYCN-A	Low suppressive and stromal; immune checkpoints	High infiltrate, high MHC
4a	High-risk/Poor	Intermediate cytotoxic	MYCN-NA >> MYCN-A	MDSCs, high stromal; immune checkpoints	Higher than 4b
4b	High-risk/Poor	Immunosuppressive	MYCN-NA >> MYCN-A	MDSCs, high stromal	Lower than 4a