Table 3. Quantification of TiLV from fish and water samples during an active outbreak in river open-cages.

Cage in Farm 1	Samples	Cq	TiLV load*	Interpretation
A	Diseased fish A1-1 (liver + spleen)	13.02	2.64 × 108	+
	Diseased fish A1-2 (liver + spleen)	30.69	2.18 × 103	+
	Diseased fish A1-3 (liver + spleen)	13.11	2.49 × 108	+
	Water sample A1	36.76	8.50 × 103	+
	Water sample A2	31.95	2.06 × 105	+
B	Diseased fish B1-1 (liver + spleen)	14.35	1.10 × 108	+
	Diseased fish B1-2 (liver + spleen)	17.49	1.37 × 107	+
	Diseased fish B1-3 (liver + spleen)	13.13	2.46 × 108	+
	Water sample B1	32.54	1.39 × 105	+
	Water sample B2	31.60	2.59 × 105	+
C	Diseased fish C1-1 (liver + spleen)	14.76	8.34 × 107	+
	Diseased fish C1-2 (liver + spleen)	13.87	1.50 × 108	+
	Water sample C1	32.71	1.24 × 105	+
	Water sample C2	31.49	2.79 × 105	+
D	Diseased fish D1-1 (liver + spleen)	36.22	5.6 × 101	+
	Diseased fish D1-2 (liver + spleen)	12.40	3.98 × 108	+
	Diseased fish D1-3 (liver + spleen)	18.67	6.26 × 106	+
	Water sample D1	35.90	1.50 × 105	+
	Water sample D2	31.19	3.40 × 105	+

Note:

Gray highlights water samples; An asterisk (*) indicates the viral copy (per reaction for 150 ng fish extracted RNA & per L of water sample); A plus (+) indicates the virus detected.