Table 4. Quantification of TiLV from fish and pond water samples from earthen ponds.
| Farm | Pond | Samples | Cq | TiLV load* | Interpretation | |
|---|---|---|---|---|---|---|
| Farm/Hatchery 2 (Active TiLV outbreak) | Fingerling pond C1 (TiLV affected pond) | Fish | Diseased F1 (liver + spleen) | 12.42 | 3.93 × 108 | + |
| Diseased F2 (liver + spleen) | 14.56 | 9.53 × 107 | + | |||
| Diseased F3 (liver + spleen) | 12.11 | 4.83 × 108 | + | |||
| Diseased F4 (liver + spleen) | 10.77 | 1.17 × 109 | + | |||
| Diseased F5 (liver) | 13.46 | 4.17 × 108 | + | |||
| Healthy looking F1 (whole fish) | 29.85 | 3.80 × 103 | + | |||
| Water | Location 1 | 39.73 | – | – | ||
| Location 2 | 33.30 | 8.41 × 104 | + | |||
| Snail | Pooled sample | − | − | − | ||
| Sludge | Pooled sample 1 | − | − | − | ||
| Pooled sample 2 | − | − | − | |||
| Fingerling pond C2 (No signs of TiLV) | Fish | Healthy looking F1 (whole fish) | − | − | − | |
| Heathy looking F2 (whole fish) | 32.88 | 5.11 × 102 | + | |||
| Water | Location 1 | 34.66 | 3.42 × 104 | + | ||
| Location 2 | 39.76 | − | − | |||
| Fingerling pond C3 (No signs of TiLV) | Fish | Healthy looking F1 (whole fish) | 37.34 | 2.6 × 101 | ||
| Healthy looking F2 (whole fish) | − | − | − | |||
| Water | Location 1 | − | − | – | ||
| Location 2 | − | − | – | |||
| Broodstock pond B1 (No signs of TiLV) | Fish | Female brood 1, Healthy looking# | 37.08 | 3.10 × 101 | ||
| Female brood 2, Healthy looking# | 35.42 | 9.50 × 101 | ||||
| Male brood 1, Healthy looking# | 38.28 | − | − | |||
| Male brood 2, Healthy looking# | 36.18 | 5.70 × 101 | ||||
| Water | Location 1 | 37.79 | 4.29 × 103 | + | ||
| Broodstock pond B2 (No signs of TiLV) | Water | Location 1 | − | − | − | |
| Location 2 | − | − | − | |||
| Broodstock pond B3 (No signs of TiLV) | Water | Location 1 | − | − | − | |
| Location 2 | − | − | − | |||
| Sewage | Water | Location 1 | 34.61 | 3.53 × 104 | + | |
| Location 2 | − | − | − | |||
| Reservoir | Water | Location 1 | − | − | − | |
| Location 2 | 37.78 | 4.32 × 103 | + | |||
| Farm 3 (Active TiLV outbreak) | Fish | Survivor F1 (spleen) | 36.45 | 4.80 × 101 | + | |
| Survivor F2 (spleen) | 37.22 | 2.88 × 101 | + | |||
| Water | Location 1 | 35.08 | 2.59× 104 | + | ||
| Location 2 | 39.03 | − | − | |||
| Location 3 | 35.90 | 1.50× 104 | + | |||
| Farm 4 (With history of TiLV outbreak) | Fish | Healthy F1 (whole fish) | − | − | − | |
| Healthy F2 (whole fish) | − | − | − | |||
| Water | Location 1 | 39.27 | − | − | ||
| Location 2 | − | − | − | |||
| Farm 5 (With history of TiLV outbreak) | Water | Location 1 | 39.18 | − | − | |
| Location 2 | 38.24 | − | − | |||
| Location 3 | − | − | − | |||
| Farm 6 (No history of TiLV outbreak) | Water | Location 1 | − | − | − | |
| Location 2 | − | − | − | |||
Note:
Gray highlights water samples; An asterisk (*) indicates the viral copy (per reaction for 150 ng fish extracted RNA & per L of water sample); #, liver, kidney, spleen, gill, gonad; A negative (−) indicates that the virus was not detected; A positive (+) indicates that the virus was detected.