Skip to main content
. 2022 Apr 21;17(4):e0257408. doi: 10.1371/journal.pone.0257408

Fig 1. Viral replication and cell death caused in ZIKV-infected macrophages.

Fig 1

Human THP-1 (A) and mouse RAW264.7 (B) cells were infected with ZIKV at 1 MOI and shown cytopathic effect (CPE) at 24 and 48 hrs p.i. (Magnification x40). Cell viability was assessed at various time points p.i. in THP-1 (C) and RAW264.7 (D) cells infected with ZIKV at various MOIs by an MTT assay. Total RNA was prepared for realtime RT-PCR to measure copy numbers of ZIKV E RNA in infected THP-1 and RAW264.7 cells. A SYBR Green approach for fold change (E) and a TaqMan approach (F) for viral RNA copy numbers were performed. (G) ZIKV E protein expression was detected in cell lysates prepared from THP-1 and RAW264.7 cells, infected with ZIKV at an MOI of 0.1, by western blot analyses. (H) Infectious viral titers (TCID50) were titrated in Vero E6 cells. The data were presented as mean ±SD and analyzed by Student’s t-test. *, P<0.05; **, P<0.01; ***, P< 0.001.