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. 2022 Apr 21;17(4):e0257408. doi: 10.1371/journal.pone.0257408

Fig 3. Blockage of cell death induced in ZIKV-infected cells by the inhibitors of programmed cell death.

Fig 3

THP-1 (A) and RAW264.7 (B) cells were pre-treated with pan-caspases inhibitor, ZVAD-FMK, or RIPK inhibitors, Nec-1s and GSK’872, followed by infection with ZIKV at 1 MOI. The cell viability was assessed at 24 and 48 hrs p.i. with an MTT assay. The data were shown as mean ±SD and analysed by unpaired Students t-test. *, P<0.05; **, P<0.01; ***, P<0.001. ns, no significance. (C) Cleavage of pro-caspase-3 and PARP in ZIKV-infected THP-1 cells as detected in a western blot analysis. (D) Phosphorylation of RIPK and MLKL in ZIKV-infected THP-1 cells.