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. 2022 Apr 21;17(4):e0257408. doi: 10.1371/journal.pone.0257408

Fig 8. Transcriptional upregulation and secretion of IL-1β and IL-18 in ZIKV-infected human and murine macrophages.

Fig 8

Total RNA was prepared at various time points p.i. from THP-1 (A-B) and RAW264.7 (C-D) cells infected with 0.1 MOI of ZIKV for realtime RT-PCR to measure mRNA transcript numbers of pro-IL-1β (A & C) and pro-IL-18 (B & D) genes. Culture medium was sampled at various time points p.i. from ZIKV-infected THP-1 (E-F) and RAW264.7 (G-H) cells for measurement of secreted IL-1β (E & G) and IL-18 (F & H) by ELISA. The experiments were performed in triplicates and the data were shown as mean+SD and analysed by unpaired Students t-test. *, P<0.05; **, P<0.01; ***, P<0.001. ns, no significance.