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. 2022 Apr 21;17(4):e0257408. doi: 10.1371/journal.pone.0257408

Fig 9. Pyroptosis was dependent on caspase-1 in ZIKV-infected human and murine macrophages.

Fig 9

Cell lysates were prepared at various time points p.i. from ZIKV-infected THP-1 (A) and RAW264.7 (B) cells which were untreated or pre-treated with VX765, a caspase-1 inhibitor, at 20μM. The lysates were analysed by western blot analyses with an anti-cleaved caspase-1 antibody. Morphological changes were observed on ZIKV-infected THP-1 (C) and RAW264.7. Arrows, swollen or ruptured cells. (D) cells, which were untreated or pre-treated with VX765, under a light microscope (Magnification x200). (E & F) Cell viability of THP-1 and RAW264.7, pre-treated with or without VX765 and followed by ZIKV infection (0.1 MOI), was measured by a CCK8 assay. (G & H) Culture medium was sampled at various time points p.i. from ZIKV-infected THP-1 and RAW264.7 cells, which were untreated or pre-treated with VX765, for measurement of secreted IL-1β by ELISA. The experiments were performed in triplicates and the data were shown as mean+SD and analyzed by unpaired Students t-test. *, P<0.05; **, P<0.01; ***, P<0.001. ns, no significance.