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. 2022 Apr 21;17(4):e0266914. doi: 10.1371/journal.pone.0266914

Table 4. Concordance of OncoFocus panel vs ActSeq panel.

OncoFocus ActSeq
Patient Gender Age Source Gene Mutation Mut Freq Mutation AA Mutation CDS VAF
H21001 Male 86 colon KRAS Q61H 0.30 p.Q61H c.183A>T 0.25
H21002 Male 79 lung KRAS G12C 0.61 p.G12C c.34G>T 0.59
H21003 Female 81 colon BRAF V600E 0.28 p.V600E c.1799T>A 0.32
H21004 Male 86 melanoma BRAF V600K 0.19 p.V600K c.1798_1799GT>AA 0.21
H21005 Female 64 Ovarian KRAS G13D 0.28 p.G13D c.38G>A 0.15
H21006 Male 76 melanoma NRAS Q61L 0.16 p.Q61L c.182A>T 0.16
H21007 Male 61 lung EGFR G719A 0.45 p.G719A c.2156G>C 0.36
H21008 Male 84 colon KRAS Q61H 0.38 p.Q61H c.183A>T 0.36
H21009 Male 76 colon KRAS G12V 0.52 p.G12V c.35G>T 0.44
H21010 Female 90 melanoma NRAS Q61R 0.32 p.Q61R c.182A>G 0.21
H21012 Female 63 colon BRAF V600E 0.19 p.V600E c.1799T>A 0.24
H21013 Female 74 melanoma BRAF V600K 0.48 p.V600K c.1798_1799GT>AA 0.51
H21014 Male 89 colon KRAS G12D 0.38 p.G12D c.35G>A 0.38
H21015 Female 70 colon KRAS Q61H 0.39 p.Q61H c.183A>C 0.38
H21016 Male 54 melanoma BRAF V600E 0.35 p.V600E c.1799T>A 0.58
H21019 Female 68 melanoma BRAF V600K 0.49 p.V600K c.1798_1799GT>AA 0.59
H21020 Male 59 colon KRAS G13D 0.22 p.G13D c.38G>A 0.17
H21021 Male 56 colon KRAS G12V 0.44 p.G12V c.35G>T 0.44
H21022 Female 77 lung EGFR L858R 0.48 p.L858R c.2573T>G 0.64
H21023 Male 62 GIST KIT V559D 0.39 p.V559D c.1676T>A 0.48
H21024 Male 79 colon KRAS G12A 0.41 p.G12A c.35G>C 0.43
H21025 Male 69 lung KRAS G13D 0.45 p.G13D c.38G>A 0.37
H21026 Male 64 colon KRAS A146T 0.52 p.A146T c.436G>A 0.42
H21027 Male 82 colon BRAF V600E 0.25 p.V600E c.1799T>A 0.24
H21029 Female 59 lung KRAS G12C 0.22 p.G12C c.34G>T 0.19
H21030 Female 63 lung EGFR E746_A750del 0.81 p.E746_A750delELREA c.2235_2249del15 0.74
H21031 Female 79 lung KRAS Q61H 0.44 p.Q61H c.183A>C 0.40
H21032 Female 65 colon KRAS A146T 0.39 p.A146T c.436G>A 0.31
H21033 Female 71 lung EGFR L858R 0.27 p.L858R c.2573T>G 0.36
H21034 Female 77 lung KRAS G12S 0.22 p.G12S c.34G>A 0.23
H21035 Female 53 lung EGFR L861Q 0.39 p.L681Q c.2582T>A 0.40
H21036 Male 66 lung KRAS G12C 0.30 p.G12C c.34G>T 0.27
H21037 Male 62 colon KRAS G12A 0.30 p.G12A c.35G>C 0.33
H21038 Female 80 colon KRAS Q61R 0.18 p.Q61R c.182A>G 0.25
H21039 Female 80 colon BRAF V600E 0.17 p.V600E c.1799T>A 0.21
H21040 Male 68 colon KRAS G12D 0.23 p.G12D c.35G>A 0.28
H21041 Female 67 lung KRAS G12C 0.59 p.G12C c.34G>T 0.62
H21042 Female 62 lung BRAF G469A 0.36 p.G469A c.1406G>C 0.40
H21043 Male 66 colon NRAS Q61K 0.45 p.Q61K c.181C>A 0.46
H21044 Male 66 lung NRAS G12C 0.34 p.G12C c.34G>T 0.33
H21045 Female 73 colon BRAF V600E 0.40 p.V600E c.1799T>A 0.44
H21046 Male 82 colon KRAS G12D 0.38 p.G12D c.35G>A 0.46
H21047 Female 74 colon BRAF V600E 0.25 p.V600E c.1799T>A 0.30
H21048 Female 63 lung KRAS G12D 0.16 p.G12D c.35G>A 0.15
H21049 Male 69 colon NRAS Q61H 0.27 p.Q61H c.183A>T 0.18