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. 2021 May 10;93(20):7422–7429. doi: 10.1021/acs.analchem.0c04749

Figure 1.

Figure 1

Schematic of compound NAC workflow. (a) A mixed DNA sample is sorted in the first NAC round using TaqMan targeting a desired sequence biomarker. Each NAC round comprises (i) DNA encapsulation with TaqMan reagents; (ii) in-droplet PCR to generate fluorescence when the target is present; (iii) sorting to select positive drops; and (iv) recovery of sorted DNA by droplet demulsification. (b) DNA recovered from the first NAC round is diluted and processed through another round consisting of the same steps (v–viii). (c) The double-enriched DNA is analyzed by qPCR to estimate enrichment and sequenced.