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. 2021 May 10;93(20):7422–7429. doi: 10.1021/acs.analchem.0c04749

Figure 3.

Figure 3

Enrichment of the ΦX 174 DNA from a background of lambda DNA with compound NAC. (a) TaqMan assays detect droplets containing ΦX 174 (green) and lambda (red) DNA. (b) The microfluidic sorter interrogates the droplets for fluorescence and sorts PCR positives. (i) Scatter plot of fluorescence vs size of drops from the first NAC round, with 0.24% positive. (ii) DNA from the first round is recovered, diluted, and processed again. (iii) Scatter plot of fluorescence vs size of drops from the second NAC round, with 0.17% positive. (c) qPCR plots for (i) single- and (ii) double-enriched DNA; based on curve shifts, single-round sorting enriches ΦX 174 by ∼150-fold and double-round sorting by ∼16,000-fold. Inset in (i) shows ΦX 174 and lambda standard curves.